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| Status |
Public on Aug 28, 2017 |
| Title |
RNA-seq gene expression profiling in resting murine B cells from WT or KMT2D knockout mice, before and after stimulation with LPS, IL4, and anti-CD180 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Resting B cells were isolated from WT or KMTD KO mice by immunomagentic depletion of with anti-CD43 beads. Gene expression use determined by RNAseq in resting B cells or B cells stimulated with LPS, IL4, and anti-mouse CD180 for 3 days.
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| |
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| Overall design |
RNAseq was used to profile unstimulated resting B cells (n=3) and B cells stimulated for 3 days (n=3).
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| |
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| Contributor(s) |
Canela A, Boss IW, Pan H, Melnick A, Nussenzweig A |
| Citation(s) |
26366710 |
| Submission date |
May 12, 2015 |
| Last update date |
Dec 16, 2019 |
| Contact name |
Yanwen Jiang |
| E-mail(s) |
yaj2001@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Street address |
413 E. 69th St. BB1462
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| SRA |
SRP058264 |
| BioProject |
PRJNA283823 |
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