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| Status |
Public on May 15, 2015 |
| Title |
Gene expression in IL-17RA-deficient and wild-type mice following Giardia muris infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To elucidate IL-17A-dependent immune mechanims involved in regulating host defense, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential of regulating protective immunity that failed to be upregulated in the absence of IL-17RA signaling. Whole small intestine tissue collected from IL-17RA-deficient and C57BL/6 (wild-type) mice was collected 2 weeks after Giardia muris infection and from uninfected controls. Expression levels of several genes that may have anti-parasitic potential (Defb1, Retnlb, Saa1, Saa2) and may be involved in parasite clearance were, on average, lower or unchanged in IL-17RA deficient compared to wild-type mice after infection.
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| Overall design |
Two weeks after Giardia muris challenge, total RNA was extracted and analyzed from tissue of the small intestines of infected IL-17RA-deficient and wild-type mice, and compared to uninfected controls. Each sample contained equal amounts of total RNA from 6 female mice which were pooled and used in the experiment.
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| Contributor(s) |
Dann SM, Eckmann L |
| Citation(s) |
26071205 |
| Submission date |
May 14, 2015 |
| Last update date |
May 10, 2018 |
| Contact name |
Sara M Dann |
| E-mail(s) |
smdann@utmb.edu
|
| Organization name |
University of Texas Medical Branch
|
| Department |
Internal Medicine
|
| Street address |
301 University Blvd
|
| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77555-0435 |
| Country |
USA |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA283945 |
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