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Series GSE6887 Query DataSets for GSE6887
Status Public on May 09, 2007
Title Gene expression profile of peripheral blood lymphocytes: comparison between melanoma patients and healthy controls
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We focused on the major peripheral blood lymphocyte populations that may be involved in anti-tumor responses and negatively impacted by cancer, specifically CD8 T cells, CD4 T cells, B cells and CD56dim natural killer cells. The pure cell subsets were stringently sorted by flow cytometry from PBMC samples. Gene expression profiles of these cell populations from melanoma patients were compared to healthy controls.
Keywords: gene expression profile
Overall design The raw data set contained 48 arrays: 6 healthy and 6 melanoma arrays for each of the 4 cell types. Two of the arrays had quality issues due to background noise and were excluded, leaving us with 46 arrays.

On each array we used Cy3 to label a pool of two RNA samples from a pair of age and gender matched stage IV (American Joint Committee on Cancer) melanoma patients or from a pair of age and gender matched healthy donors. We used Cy5 to label the Total Lymphocyte Reference (TLR) RNA. The TLR is a common reference specifically created for this study using the total peripheral lymphocyte fraction from 20 healthy donors.
Contributor(s) Critchley-Thorne RJ, Yan N, Nacu S, Weber J, Holmes SP, Lee PP
Citation(s) 17488182
Submission date Jan 28, 2007
Last update date Dec 06, 2012
Contact name Peter P. Lee
Phone 650-498-7942
Fax 650-736-0974
Organization name Stanford University
Department Department of Medicine
Street address CCSR room 1155, 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL887 Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version)
Samples (46)
GSM158372 B_cell_pool_healthy_H01+H02
GSM158512 B_cell_pool_healthy_H03+H04
GSM158513 B_cell_pool_healthy_H05+H06
BioProject PRJNA98563

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Supplementary file Size Download File type/resource
GSE6887_RAW.tar 3.3 Mb (http)(custom) TAR

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