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| Status |
Public on May 15, 2017 |
| Title |
Adipocyte-derived Exosomal miRNAs from Subcutaneous Adipose Tissue: A Novel Mechanism for Obesity-Related Disease |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Affymetrix miRNA 3.0 array profiling of adipocyte-derived exosomes from obese and lean human subjects. We used miRNA arrays to profile exosomes shed from obese and lean human subcutaneous fat that was cultured for 60 minutes.
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| Overall design |
Human obese and lean subcutaneous fat were surgically acquired, dissected, and promptly cultured for 60 minutes. We used the culture supernatants for exosome purification and isolation using ExoQuick-TC Precipitation Solution.
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| Contributor(s) |
Barberio M, Nadler E, Freishtat RJ, Ferrante SC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 14, 2015 |
| Last update date |
Jul 27, 2018 |
| Contact name |
Robert Freishtat |
| E-mail(s) |
rfreishtat@childrensnational.org
|
| Organization name |
Children's National Medical Center
|
| Department |
Center for Genetic Medicine
|
| Street address |
111 Michigan Avenue NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
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| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA283971 |
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