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| Status |
Public on Jun 17, 2015 |
| Title |
Tumor exosome integrins determine organotropic metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stephen Paget first proposed, in 1889, that organ distribution of metastases is a non-random event, yet metastatic organotropism remains one of the greatest mysteries in cancer biology. Here, we demonstrate that exosomes released by lung-, liver- and brain-tropic tumor cells fuse preferentially with resident cells at their predicted destination, such as fibroblasts and epithelial cells in the lung, Kupffer cells in the liver, and endothelial cells in the brain. We found that exosome homing to organ-specific cell types prepares the pre-metastatic niche and that treatment with exosomes derived from lung tropic models can redirect metastasis to the lung. Proteomic profiling of exosomes revealed distinct integrin expression patterns associated with each organ-specific metastasis. Whereas exosomal integrins α6β4 and α6β1 were associated with lung metastasis, exosomal integrins αvβ5 and αvβ3 were linked with liver and brain metastases, respectively. Targeting α6β4 and αvβ5 integrins decreased exosome uptake and metastasis in the lung and liver, respectively. Importantly, we demonstrate that exosome uptake activates a cell-specific subset of S100 family genes, known to support cell migration and niche formation. Finally, our clinical data indicate that integrin-expression profiles in circulating plasma exosomes from cancer patients could be used to predict organ-specific metastasis.
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| Overall design |
Education of human von Kupffer cells in vitro with human pancreatic cancer exosomes
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| Contributor(s) |
Hoshino A, Costa-Silva B, Shen T, Rodrigues G, Hashimoto A, Tesic Mark M, Molina H, Kosaka S, Di Giannatale A, Ceder S, Singh S, Williams C, Ararso Y, Zhang T, Kure EH, Mallya K, Batra SK, Vinagolu RK, Fodstad O, Muller V, Pantel K, Bissell MJ, Ghajar C, Garcia BA, Kang Y, Matei I, Peinado H, Bromberg J, Lyden D |
| Citation(s) |
25985394, 26524530 |
| Submission date |
May 15, 2015 |
| Last update date |
Jul 26, 2019 |
| Contact name |
David Lyden |
| E-mail(s) |
dcl2001@med.cornell.edu
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| Organization name |
Weill Medical College of Cornell University
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| Department |
Departments of Pediatrics, Cell & Developmental Biology
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| Street address |
413 E. 69th St., 12th Floor, BB-1251
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA284109 |
| SRA |
SRP058375 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68919_Processed_BxPc3exo_vs_Beta5KD.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE68919_Processed_Control_vs_BxPc3exo.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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