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| Status |
Public on May 09, 2016 |
| Title |
Mesenchymal stromal cells perturb human monocyte in vitro differentiation into dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Monocytes cultured in media containing GM-CSF and IL4 for 5 days normally differentiate into immature dendritic cells which, upon further stimulation with LPS for an additional 2 days, acquire a mature phenotype. In the present study, we evaluated the effect of human cord-blood mesenchymal stem cells (MSC) on human monocyte differentiation into immature and mature DCs by examining the trancriptional profile of the cells that were obtained after both the differentiation and maturation stages.
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| Overall design |
This is a six condition experiment (iDC, iDC-MSC, mDC, mDC-MSCd, mDC-MSCm, mDC-MSCdm) where each condition is in biological triplicates, that is 3 donors (A, B, C) per condition. iDC are cells obtained after 5-day differentiation of monocytes, otherwise known as immature DCs. iDC-MSC are the cells obtained at the end of the 5-day differentiation phase of monocytes, during which MSC were also present. mDC are cells obtained after a 2-day maturation of iDC with LPS, also known as mature DCs. mDC-MSC are cells obtained after MSC have been present during only the differentiation (mDC-MSCd) phase, only the maturation (mDC-MSCm) phase or during both the differentiation and maturation phases (mDC-MSCdm).
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| Contributor(s) |
Selleri S, Bifsha P, Haddad E |
| Citation(s) |
27070086 |
| Submission date |
May 15, 2015 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Panojot Bifsha |
| E-mail(s) |
panojot.bifsha@gmail.com
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| Organization name |
CHU Sainte-Justine
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| Department |
Pediatrics
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| Street address |
3175 Chemin de la Côte Ste-Catherine
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| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3T 1C5 |
| Country |
Canada |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (18)
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| GSM1688825 |
Monocyte_Differentiation(MSC)_donorA |
| GSM1688826 |
Monocyte_Differentiation(MSC)_donorB |
| GSM1688827 |
Monocyte_Differentiation(MSC)_donorC |
| GSM1688828 |
Monocyte_Differentiation_Maturation_donorA |
| GSM1688829 |
Monocyte_Differentiation_Maturation_donorB |
| GSM1688830 |
Monocyte_Differentiation_Maturation_donorC |
| GSM1688831 |
Monocyte_Differentiation(MSC)_Maturation_donorA |
| GSM1688832 |
Monocyte_Differentiation(MSC)_Maturation_donorB |
| GSM1688833 |
Monocyte_Differentiation(MSC)_Maturation_donorC |
| GSM1688834 |
Monocyte_Differentiation(MSC)_Maturation(MSC)_donorA |
| GSM1688835 |
Monocyte_Differentiation(MSC)_Maturation(MSC)_donorB |
| GSM1688836 |
Monocyte_Differentiation(MSC)_Maturation(MSC)_donorC |
| GSM1688837 |
Monocyte_Differentiation_Maturation(MSC)_donorA |
| GSM1688838 |
Monocyte_Differentiation_Maturation(MSC)_donorB |
| GSM1688839 |
Monocyte_Differentiation_Maturation(MSC)_donorC |
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| Relations |
| BioProject |
PRJNA284166 |
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