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Series GSE6909 Query DataSets for GSE6909
Status Public on Aug 30, 2007
Title Differential gene expression in placentae (E10.5) from adra2bKO and adra2bWT mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary alpha2-adrenoceptors are essential presynaptic regulators of norepinephrine release from sympathetic nerves. Previous studies in mice with targeted deletions in the three alpha2-adrenoceptor genes have indicated that these receptors are essential for embryonic development. In the present study, we searched for the alpha2-adrenoceptor subtype(s) involved in placental development and its molecular mechanism using mice carrying targeted deletions in alpha2-adrenoceptor genes.
Congenic alpha2B-adrenoceptor-deficient mice (Adra2b-/-) developed a defect in fetal and maternal vessel formation in the placenta labyrinth at embryonic day E10.5. This defect was accompanied by reduced endothelial cell proliferation and decreased ERK1/2 phosphorylation levels in Adra2b-/- as compared with Adra2b+/+ placenta. Microarray analysis of wild-type and mutant placentae (maternal genotype Adra2b+/-) revealed 179 genes which were significantly up- or downregulated >1.5-fold in alpha2B-deficient placenta. The type 1 receptor for vascular endothelial growth factor (Flt1), which is coexpressed with alpha2B-adrenoceptors in spongiotrophoblast and giant cells of the placenta, was found to be 2.3-fold upregulated in alpha2B-deficient placenta. Neutralization of Flt1 and its soluble splice variant sFlt1 by a specific antibody in vivo prevented the vascular defect in alpha2B-deficient placenta at E10.5. Thus, alpha2B-adrenoceptors are essential to suppress antiangiogenic (s)Flt1 in spongiotrophoblasts to control the coordinated formation of a vascular labyrinth of fetal and maternal blood vessels in the murine placenta during development.
Keywords: Comparative expression profiling
Overall design Comparative transcriptome analysis was determined using the GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA, USA). Six microarrays from three wild-type and three adra2b deficient mice were performed.
Contributor(s) Gilsbach R, Muthig V, Ivacevic T, Hein L
Citation(s) 17673674
Submission date Jan 30, 2007
Last update date Feb 11, 2019
Contact name Ralf Gilsbach
Organization name Institute of Pharmacology
Lab Prof. Dr. Lutz Hein
Street address Albertstr. 25
City Freiburg
ZIP/Postal code 79104
Country Germany
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM159345 Placenta Adra2bKO 1
GSM159346 Placenta Adra2bKO 2
GSM159347 Placenta Adra2bKO 3
BioProject PRJNA98523

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Supplementary file Size Download File type/resource
GSE6909_RAW.tar 35.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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