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Series GSE6934 Query DataSets for GSE6934
Status Public on Feb 03, 2007
Title Transcriptional comparison between whole kidneys from E14.5 Wnt4 mutants and wildtype mice (Mouse430_2 platform). (GUDMAP Series ID: 13)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Our laboratory's interest is in understanding the molecular principles that underlie the regional organization of the mammalian metanephric kidney. Our goal is to generate a detailed spatial map of the cellular expression of selected regulatory genes during mammalian kidney development. The goal of this study is to identify a population of genes that are enriched in the renal vesicle (RV) and its derivatives using Wnt4 mutants.
Keywords: mutant comparison
Overall design Wnt4 is required for renal vesicle (RV) induction. Therefore, RV and the derivatives (s-shaped body and eventually the mature nephron) are missing in Wnt4 mutants. At E14.5, these structures are present in wildtype kidneys. Transcriptional profile comparison between E14.5 wildtype and Wnt4 mutants therefore identify genes expressed in the RV and derivatives. Minimally pooled kidney samples were used as single biological replicates. Total RNA was isolated from these pools and subjected to a single round of amplification for use on Affymetrix arrays.
Contributor(s) McMahon AP, Valerius MT
Citation(s) 19501082
Submission date Feb 01, 2007
Last update date Feb 11, 2019
Contact name GUDMAP Developers
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM159983 wnt4_430_wt1
GSM159984 wnt4_430_wt2
GSM159985 wnt4_430_mut1
BioProject PRJNA98109

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6934_RAW.tar 25.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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