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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 10, 2017 |
| Title |
RNA-sequencing of EpH4 and EpRas cells treated with Etv4 and Etv5 siRNAs and/or TGF-beta. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-seq analysis in mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) to analyze the mechanisms of transcriptional regulation during TGF-beta-induced EMT.
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| Overall design |
RNA-seq was perfomed using EpH4 and EpRas cells transfected with Etv4 and Etv5 siRNAs and stimulated with 1 ng/ml TGF-beta or left untreated for 48 h.
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| Contributor(s) |
Arase M, Tamura Y, Kawasaki N, Isogaya K, Nakaki R, Mizutani A, Tsutsumi S, Aburatani H, Miyazono K, Koinuma D |
| Citation(s) |
28446749 |
| Submission date |
May 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
d-koinuma@umin.ac.jp
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| Organization name |
University of Tokyo
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| Department |
Pathology
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| Street address |
Hongo 7-3-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (14)
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| GSM2385535 |
RNA-seq, EpH4, untreated, 48 h, replicate 1 |
| GSM2385536 |
RNA-seq, EpH4, untreated, 48 h, replicate 2 |
| GSM2385537 |
RNA-seq, EpH4, TGFB treated, 48 h, replicate 1 |
| GSM2385538 |
RNA-seq, EpH4, TGFB treated, 48 h, replicate 2 |
| GSM2385539 |
RNA-seq, EpRas, untreated, 48 h, replicate 1 |
| GSM2385540 |
RNA-seq, EpRas, untreated, 48 h, replicate 2 |
| GSM2385541 |
RNA-seq, EpRas, TGFB treated, 48 h, replicate 1 |
| GSM2385542 |
RNA-seq, EpRas, TGFB treated, 48 h, replicate 2 |
| GSM2385543 |
RNA-seq, EpRas, si Negative control, 48 h |
| GSM2385544 |
RNA-seq, EpRas, si Negative control, TGFB treated, 48 h |
| GSM2385545 |
RNA-seq, EpRas, si Etv4_1 and Etv5_1, 48 h |
| GSM2385546 |
RNA-seq, EpRas, si Etv4_1 and Etv5_1, TGFB treated, 48 h |
| GSM2385547 |
RNA-seq, EpRas, si Etv4_2 and Etv5_2, 48 h |
| GSM2385548 |
RNA-seq, EpRas, si Etv4_2 and Etv5_2, TGFB treated, 48 h |
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| This SubSeries is part of SuperSeries: |
| GSE69387 |
Dynamics of chromatin accessibility during TGF-beta-induced EMT of Ras-transformed mammary gland epithelial cells |
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| Relations |
| BioProject |
PRJNA285319 |
| SRA |
SRP058847 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69363_genes.fpkm_tracking_3-8_80.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| GSE69363_genes.fpkm_tracking_9-16_80.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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