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| Status |
Public on May 29, 2015 |
| Title |
SETDB1 Represses Endogenous and Exogenous Retroviruses in B Lymphocytes [array] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome stability relies on epigenetic mechanisms that enforce repression of endogenous retroviruses (ERVs). Current evidence suggests that distinct chromatin-based mechanisms repress ERVs in cells of embryonic origin (histone methylation-dominant) versus more differentiated cells (DNA methylation-dominant). However, the latter aspect of this model has not been tested. Remarkably, and in contrast to the prevailing model, we find that repressive histone methylation catalyzed by the enzyme SETDB1 is critical for suppression of specific ERV families and exogenous retroviruses in committed B-lineage cells from adult mice. The profile of ERV activation in SETDB1-deficient B cells is distinct from that observed in corresponding embryonic tissues, despite the loss of repressive chromatin modifications at all ERVs. We provide evidence that, upon loss of SETDB1, ERVs are activated in a lineage-specific manner depending on the set of transcription factors available to target proviral regulatory elements. These findings have important implications for genome stability in somatic cells, as well as the interface between epigenetic repression and viral latency.
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| Overall design |
Expression profiling in Setdb1 C/C and Setdb1 D/D pro-B cells either directly from the bone marrow or bcl2-transgenic pro-B cells cultured in IL-7 for four days
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| Contributor(s) |
Collins PL, Kyle K, Egawa T, Shinkai Y, Oltz EM |
| Citation(s) |
26100872 |
| Submission date |
May 29, 2015 |
| Last update date |
Aug 28, 2015 |
| Contact name |
Patrick Collins |
| E-mail(s) |
patlennycollins@yahoo.com
|
| Organization name |
Washington University
|
| Department |
Pathology and Immunology
|
| Lab |
Oltz
|
| Street address |
1217 Oakleaf dr
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63119 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10740 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE69504 |
SETDB1 Represses Endogenous and Exogenous Retroviruses in B Lymphocytes |
|
| Relations |
| BioProject |
PRJNA285334 |
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