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Series GSE6939 Query DataSets for GSE6939
Status Public on Aug 01, 2007
Title CD4+ T cells gene-transduced with AML1, wild type Foxp3, and a Foxp3 mutant defective in binding to AML1
Organism Mus musculus
Experiment type Expression profiling by array
Summary To clarify how Foxp3 regulates its target genes, we performed co-immunoprecipitation experiments and found that Foxp3 physically bound to AML1/Runx1 (Ono, M. et al, Nature, 2007). In this series of study, we compared gene regulations by AML1, wild type Foxp3, and a Foxp3 mutant with defective binding to AML1.
Keywords: Retroviral gene-transduction into primary CD4+ T cells
 
Overall design CD4+ naive T cells were activated and retrovirally gene-transduced with either empty vector (pMCsIg), or AML1-, wild type Foxp3-, or an AML1-non binding mutant Foxp3-encoding vectors. Sixty hours after transfection, 5 x 10^6 GFP-expressing cells were sorted and total RNA was extracted. One cycle target labelling protocol was used for labelling RNA with biotin.
 
Contributor(s) Ono M, Yaguchi H, Ohkura N, Kitabayashi I, Nagamura Y, Nomura T, Miyachi Y, Tsukada T, Sakaguchi S
Citation(s) 17377532
Submission date Feb 02, 2007
Last update date Feb 11, 2019
Contact name Masahiro Ono
E-mail(s) m.ono@ucl.ac.uk
Organization name Institute of Child Health
Department Immunobiology Unit
Street address 30 Guilford Street
City London
ZIP/Postal code WC1N 1EH
Country United Kingdom
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM160037 empty vector
GSM160039 AML1
GSM160041 wild type Foxp3
Relations
BioProject PRJNA98119

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6939_RAW.tar 13.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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