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| Status |
Public on Dec 31, 2015 |
| Title |
Transcriptome study of Caco-2 human intestinal cells following exposure to empty or S-nitrosoglutathione-loaded polymeric nanoparticles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To further study the transcriptome of Caco-2 human colon epithelial-like cells after exposure to S-nitrosoglutathione (GSNO, 1.4 μM), or Eudragit RL PO polymeric nanoparticles (NP-ERL, 50 μg/mL) or GSNO loaded nanoparticles (NP-GSNO, 50 μg/mL corresponding to (1.4 μM GSNO) we investigate whole genome microarray to identify genes regulates by exposure or not to GSNO (1.4 μM) or Eudragit RL PO polymeric nanoparticles (NP-ERL, 50 μg/mL) or GSNO loaded nanoparticles (NP-GSNO, 50 μg/mL corresponding to (1.4 μM GSNO).
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| Overall design |
Changes in gene expression in Caco-2 cells incubated without (control) or with GSNO or nanoparticles for 4 h, were measured. Four biological replicates were performed as controls: S46_1_4 ; S46_1_3 ; S35_1_4 ; S35_1_3. Four biological replicates were performed for each conditions : wtih GSNO (1.4 µM) exposed cells (S46_2_2 ; S46_2_1 ; S35_2_2 ; S35_2_1), with NP-ERL (50 μg/mL) exposed cells (S46_1_2 ; S46_1_1 ; S35_1_2 ; S35_1_1) with NP-GSNO (50 μg/mL corresponding to 1.4 µM GSNO) exposed cells (S46_2_4 ; S46_2_3 ; S35_2_4 ; S35_2_3)
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| Contributor(s) |
Puisney C, Safar R, Joubert OR, Ferrari L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 04, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Olivier Joubert |
| E-mail(s) |
olivier.joubert@univ-lorraine.fr
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| Phone |
0372742694
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| Organization name |
Université de lorraine
|
| Department |
Dpt 4
|
| Lab |
Nanomatériaux et santé
|
| Street address |
2 allée guinier
|
| City |
nancy |
| ZIP/Postal code |
54011 |
| Country |
France |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (16)
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| GSM1703772 |
Caco-2_Control_rep4 |
| GSM1703773 |
Caco-2_4h_1.4 µM_of_GSNO_rep1 |
| GSM1703774 |
Caco-2_4h_1.4 µM_of_GSNO_rep2 |
| GSM1703775 |
Caco-2_4h_1.4 µM_of_GSNO_rep3 |
| GSM1703776 |
Caco-2_4h_1.4 µM_of_GSNO_rep4 |
| GSM1703777 |
Caco-2_4h_50µg/mL_of_GSNO (1.4 µM) loaded polymeric nanoparticles (Eudragit® RLPO)_rep1 |
| GSM1703778 |
Caco-2_4h_50µg/mL_of_GSNO (1.4 µM) loaded polymeric nanoparticles (Eudragit® RLPO)_rep2 |
| GSM1703779 |
Caco-2_4h_50µg/mL_of_GSNO (1.4 µM) loaded polymeric nanoparticles (Eudragit® RLPO)_rep3 |
| GSM1703780 |
Caco-2_4h_50µg/mL_of_GSNO (1.4 µM) loaded polymeric nanoparticles (Eudragit® RLPO)_rep4 |
| GSM1703781 |
Caco-2_4h_50µg/mL_of_polymeric nanoparticles (Eudragit® RLPO)_rep1 |
| GSM1703782 |
Caco-2_4h_50µg/mL_of_polymeric nanoparticles (Eudragit® RLPO)_rep2 |
| GSM1703783 |
Caco-2_4h_50µg/mL_of_polymeric nanoparticles (Eudragit® RLPO)_rep3 |
| GSM1703784 |
Caco-2_4h_50µg/mL_of_polymeric nanoparticles (Eudragit® RLPO)_rep4 |
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| Relations |
| BioProject |
PRJNA285840 |
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