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Series GSE6957 Query DataSets for GSE6957
Status Public on Jul 19, 2007
Title Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers (430)
Organism Mus musculus
Experiment type Expression profiling by array
Summary The ability to purify to homogeneity a population of hepatic progenitor cells from adult liver is critical for their characterization prior to any therapeutic application. As a step in this direction, we have utilized gene profiling of a bipotential liver cell line from dpc 14 mouse embryonic liver to catalog genes expressed by liver progenitor cells. These cells, known as Bipotential Mouse Embryonic Liver (BMEL) cells, proliferate in an undifferentiated state and are capable of differentiating into hepatocyte-like and cholangiocyte-like cells in vitro. Upon transplantation, BMEL cells are capable of differentiating into hepatocytes and cholangiocytes in vivo. Microarray analysis of gene expression in the 9A1 and 14B3 BMEL cell lines grown under proliferating and differentiating conditions was used to identify cell surface markers preferentially expressed in the bipotential undifferentiated state. This analysis revealed that proliferating BMEL cells express many genes involved in cell cycle regulation whereas differentiation of BMEL cells by cell aggregation causes a switch in gene expression to functions characteristic of mature hepatocytes. In addition, microarray data and protein analysis indicated that the Notch signaling pathway could be involved in maintaining BMEL cells in an undifferentiated stem cell state. Using GO annotation, a list of cell surface markers preferentially expressed on undifferentiated BMEL cells was generated. One marker, Cd24a, is specifically expressed on progenitor oval cells in livers of DDC treated animals. We therefore consider Cd24a expression a candidate molecule for purification of hepatic progenitor cells.
Keywords: cell type comparison
Overall design RNA was extracted from two independently isolated BMEL cell lines (9A1 and 14B3) after culture under three conditions (basal, aggregate 1 day, and aggregate 5 days). Duplicate biological replicates were collected for each cell line:culture condition combination for a total of 12 samples. Samples were biotin-labeled, hybridized to mouse 430 2.0 chips, and scanned according to established Affymetrix protocols.
Contributor(s) Ochsner SA, Strick-Marchand H, Venable S, Dean A, Wilde M, Weiss MC, Darlington GJ
Citation(s) 17641245
Submission date Feb 05, 2007
Last update date Feb 11, 2019
Contact name Scott Andrew Ochsner
Phone 713-798-6227
Organization name Baylor College of Medicine
Department Molecular and Cellular Biology
Lab SPP: Signaling Pathways Project
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM160432 basal culture, 9A1, rep 1 (430)
GSM160433 basal culture, 9A1, rep 2 (430)
GSM160434 basal culture, 14B3, rep 1 (430)
This SubSeries is part of SuperSeries:
GSE6966 Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers.
BioProject PRJNA104127

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Supplementary file Size Download File type/resource
GSE6957_RAW.tar 46.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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