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Series GSE69611 Query DataSets for GSE69611
Status Public on Jun 06, 2015
Title Splenic gene expression profile of mice immunized with an anti-Fasciola hepatica vaccine candidate: insights into the immunological mechanisms leading to protection
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fasciolosis remains an important food-borne tremaode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs as well as the molecular basis involved in acquiring protection is extremely important in designing and selecting new vaccine candidates. The present study gives a first report of microarray-based technology for describing changes in splenic gene expression profile for mice immunised with a highly effective, protection-inducing,multi-epitope, subunit-based, chemically-synthesised vaccine candidate. Female CD1 mice were immunised with synthetic peptides containing B- and T-cell epitopes as novel vaccine candidates and challenged with Fasciola hepatica metacercariae. Spleen RNA from mice immunised with the highest protection-inducing synthetic peptides was isolated, amplified and labelled using Affymetrix standardised protocols. The Ingenuity Pathway Analysis tool was used to annotate bio-functions and constructing and visualising molecular interaction networks. Immunising mice with a combination of three peptides containing T-cell epitopes induced high protection against experimental challenge according to survival rates and hepatic damage scores. It also induced differential expression of 820 genes; 168 being up-regulated and 652 down-regulated. Functional study revealed changes in pathways related to nitric oxide and reactive oxygen species production, IL-12 signalling and production in macrophages and IL-8 signalling with up-regulation of S100A8, MMP9 and CXCR2 genes. The data obtained in the present study provided us with a more comprehensive overview concerning the possible molecular pathways implied in inducing protection against Fasciola hepatica in a murine model
 
Overall design Three mice were used as healthy controls and remained untreated along the experiment. Three mice were subcutaneously immunised with our vaccine candidate on day 0, and two booster doses were applied on days 14 and 28, respectively. Two-weeks after the last immunisation, all of the mice used in the present study (including controls) were humanely euthanised by intraperitoneal injection of pentobarbital (60mg/Kg) and necropsied for spleen recovery and RNA isolation using standardised protocols. Splenic gene expression profile was compared between healthy and immunised groups.
 
Contributor(s) Rojas-Caraballo J, Lopez-Aban J, Moreno-Perez DA, Vicente B, Fernandez-Soto P, del Olmo E, Patarroyo MA, Muro A
Citation(s) 28114888
Submission date Jun 05, 2015
Last update date Jul 10, 2019
Contact name Jose Rojas-Caraballo
E-mail(s) jvrojasc@gmail.com
Organization name University of Salamanca
Department Molecular Parasitology and Immunology
Street address C/Licenciado Mendez Nieto s/n
City Salamanca
State/province Salamanca
ZIP/Postal code 37007
Country Spain
 
Platforms (1)
GPL19192 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: GATExplorer_Ensembl v57]
Samples (6)
GSM1704718 mouse untreated, biological rep1
GSM1704719 mouse untreated, biological rep2
GSM1704720 mouse untreated, biological rep3
Relations
BioProject PRJNA285982

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Supplementary file Size Download File type/resource
GSE69611_RAW.tar 33.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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