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| Status |
Public on Jun 09, 2015 |
| Title |
The C-terminal region of Xpc is dispensable for the transcriptional activity of Oct3/4 in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The transcription factor Oct3/4 is essential to maintain pluripotency in mouse embryonic stem (ES) cells. It was reported that the Xpc DNA repair complex is involved in this process. Here we examined the role of Xpc on the transcriptional activation of the target genes by Oct3/4 using the inducible knockout strategy. We found that the removal of the C-terminal region of Xpc, including the interaction sites with Rad23 and Cetn2, showed faint impact on the gene expression profile of ES cells and the functional Xpc-ΔC ES cell lines retained proper gene expression profile as well as pluripotency to contribute chimeric embryos. These data indicated that the C-terminal region of Xpc is dispensable for the transcriptional activity of Oct3/4 in mouse ES cells.
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| Overall design |
An inducible knockout ES cell line of Xpc with the Cre-loxP system was generated and gene expression was measured without Xpc deletion, after 4 days of Xpc deletion or constitutive knocked out cells. Each sample was prepared in triplicate.
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| Contributor(s) |
Ito S, Yamane M, Ohtsuka S, Niwa H |
| Citation(s) |
24607542 |
| Submission date |
Jun 08, 2015 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Hitoshi Niwa |
| E-mail(s) |
niwa@kumamoto-u.ac.jp
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| Organization name |
RIKEN
|
| Department |
Center for Developmental Biology
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| Lab |
Laboratory for Pluripotent Stem Cell Studies
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| Street address |
2-2-3 Minatojima-minamimachi,Chuo-ku
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| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA286075 |
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