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| Status |
Public on Sep 01, 2015 |
| Title |
Expression Data from Memory P14 Tg CD8 T cells after either saline mock-infection, Pichinde Virus Infection (bystander infection) or Pichinde Virus infection with daily treatment with anti-CD122 (clone TM-b1) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Exposure to inflammatory cytokines driven by bystander cytokines can have profound effects on the function of memory CD8 T cells. Here we transferred a 1-5X10^4 of P14 TCR-tg T cells (specific for gp33-41 of LCMV) on day -1 followed by infection with 2X10^5 PFU of LCMV Armstrong ip to generate memory P14 populations. Mice were rested for >50 days before further challenge. Mice containing memory P14 populations were either mock-infected with saline, infected with 2X10^6 Pichinde Virus ip (no cross-reactivity wtih LCMV gp33-41) or infected with 2X10^6 Pichinde Virus ip together with daily injections of 200 micrograms of anti-CD122 antibody (clone TM-b1). On day 4 following indicated treatments P14s were flow sorted and RNA was extracted from 1X10^6 cells using an RNeasy kit (Qiagen). Each group had 3 biological replicates. Transcriptomes were compared by DAVID analysis (p<0.01, Fold-Change > 1.5)
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| Overall design |
3 biological replicates per group. Groups included P14 from mock-infected mice, P14 from Pichinde virus infected mice and P14 from Pichinde virus and anti-cd122 treated mice.
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| Contributor(s) |
Richer MJ, Bair T, Hauser G, Boes MK, Harty JT |
| Citation(s) |
26241055 |
| Submission date |
Jun 11, 2015 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Martin Richer |
| E-mail(s) |
martin.j.richer@mcgill.ca
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| Organization name |
McGill University
|
| Street address |
406 Duff Medical Building, 3775 University street
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 2B4 |
| Country |
Canada |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA286777 |
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