|
| Status |
Public on Jun 12, 2015 |
| Title |
A biphasic epigenetic switch controls immunoevasion, virulence and niche adapatation in non-typeable Haemophilus influenzae |
| Organism |
Haemophilus influenzae |
| Experiment type |
Expression profiling by array
|
| Summary |
Non-typeable Haemophilus influenzae (NTHi) contains an N6-adenine DNA-methyltransferase (ModA), that is subject to phase variable expression (random ON/OFF switching). Five modA alleles, modA2, 4, 5, 9 and 10, account for over two-thirds of clinical otitis media isolates surveyed. Single Molecule Real Time (SMRT) methylome analysis identified the DNA recognition motifs for all five of these modA alleles. Phase variation of these alleles regulated multiple proteins, including vaccine candidates. ON/OFF switching of modA alleles resulted in differential regulation of key virulence phenotypes, such as antibiotic resistance (modA2, 5, 10), biofilm formation (modA2) and immunoevasion (modA4). Analysis of the modA2 strain, 723, in the chinchilla model of otitis media showed a clear selection for switching from modA2OFF to ON in the middle ear. This is the first report of a biphasic epigenetic switch controlling bacterial virulence, immunoevasion and niche adaptation in an animal model system
|
| |
|
| Overall design |
Direct comparison of biological triplicates of wild type and mutant strains
|
| |
|
| Contributor(s) |
Srikhanta YN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 12, 2015 |
| Last update date |
Jun 12, 2015 |
| Contact name |
Sean M Grimmond |
| E-mail(s) |
s.grimmond@imb.uq.edu.au
|
| Phone |
61 7 3346 2057
|
| Organization name |
Institute of Molecular Bioscience, Uni of QLD
|
| Department |
Genomics and Bioinformatics
|
| Lab |
Expression Genomics Lab
|
| Street address |
Research Rd
|
| City |
St Lucia |
| State/province |
QLD |
| ZIP/Postal code |
4027 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL20324 |
TIGR Haemophilus influenzae custom array |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA286882 |
Less...
More...