|
| Status |
Public on Sep 28, 2017 |
| Title |
MCPyV ST, MAX and the TRRAP complex cooperate to bind to Transcription Start Sites |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To determine if MCPyV ST was recruited to chromatin together with MAX and the TRRAP complex, we performed chromatin immunoprecipitation (ChIP) using the validated antibodies to MCPyV ST produced in our lab, HA tagged ST, MAX and EP400 followed by next generation sequencing. De novo DNA motif analysis revealed that the canonical E-box MYC target sequence was the most frequently observed motif. Metagene analysis revealed that antibodies to MAX, EP400, ST (Ab5) and HA tagged ST showed strong enrichment in transcription start site (TSS). H3K4me3 ChIP-seq confirmed that the peaks enriched with antibodies to MAX, EP400 and ST all centered on the H3K4me3 peaks with a high degree of overlap.
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| |
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| Overall design |
Examination of the hypothesis that transcripton factors Max and TRRAP complex bind same DNA loci as a complex.
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| |
|
| Contributor(s) |
Cheng J, Zeid R, Westerling T |
| Citation(s) |
29028833 |
| Submission date |
Jun 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jingwei Cheng |
| E-mail(s) |
jingweic@gmail.com
|
| Phone |
617-632-4797
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
James A. DeCaprio
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE69878 |
MCPyV ST activates Max target genes by recruiting TRRAP/EP400 complex |
|
| Relations |
| BioProject |
PRJNA287016 |
| SRA |
SRP059497 |
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