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Series GSE6998 Query DataSets for GSE6998
Status Public on Feb 09, 2007
Title Expression profiling of developmental and regenerating liver in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Normal adult liver is uniquely capable of renewal
and repair after injury. Whether this response
represents simple hyperplasia of various liver elements
or requires recapitulation of the genetic program of
the developing liver is not known. To study these possibilities,
we examined transcriptional programs of
adult liver after partial hepatectomy and contrasted
these with developing embryonic liver. Principal component
analysis demonstrated that the time series of
gene expression during liver regeneration does not segregate
according to developmental transcription patterns.
Gene ontology analysis revealed that liver restoration
after hepatectomy and liver development differ
dramatically with regard to transcription factors
and chromatin structure modification. In contrast, the
tissues are similar with regard to proliferationassociated
genes. Consistent with these findings, realtime
polymerase chain reaction showed transcription
factors known to be important in liver development
are not induced during liver regeneration. These three
lines of evidence suggest that at a transcriptional level,
restoration of liver mass after injury is best described
as hepatocyte hyperplasia and not true regeneration.
We speculate this novel pattern of gene expression may
underlie the unique capacity of the liver to repair itself
after injury.
Keywords: time course
 
Overall design In order to elucidate the molecular similarities between regenerating and developing liver, we performed high-density microarray analysis using Affymetrix MG 430 2.0 chips for targets at 0, 1, 2, 6, 12, 18, 24, 30, 48, and 72 hours after partial hepatectomy and at 10.5, 11.5, 12.5, 13.5, 14.5, and 16.5 days post conception (dpc).

Each experimental time point is represented by two separate samples, each consisting of at least 3 pooled tissues from different animals. For example, 6 hepatectomies were performed for the 1 hour post-hepatectomy time point. Time 0 is used as control.
 
Contributor(s) Otu HH, Naxerova K, Ho K, Can H, Nesbitt N, Libermanna TA, Karp SJ
Citation(s) 17227769
Submission date Feb 09, 2007
Last update date Feb 11, 2019
Contact name Hasan Huseyin Otu
E-mail(s) hotu@bidmc.harvard.edu
Organization name Harvard Medical School
Department Medicine
Lab BIDMC Genomics Center
Street address 4 Blackfan Circle Rm. 238
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (32)
GSM161108 baseline sample at T0, rep1
GSM161109 baseline sample at T0, rep2
GSM161110 regeneration sample at T1, rep1
Relations
BioProject PRJNA99277

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6998_RAW.tar 148.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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