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| Status |
Public on Aug 01, 2015 |
| Title |
Mouse ESC-derived NSPCs NFIA OE (GE) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Since the NFI transcription factors have been shown to be key regulators of gliogenesis, we utilized this pathway to identify miRNAs involved in the regulation of the neurogenesis-to-gliogenesis switch by neural stem/progenitor cells (NSPCs).
We focused on miRNAs with expression levels that were differentially regulated downstream of NFIA, and established a mouse embryonic stem cell (ESC) line that expresses NFIA in a doxycycline (Dox)-dependent manner.
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| Overall design |
NFIA-overexpressing (OE) and control NSPCs (neurospheres) derived from ESCs were purified from their mixed cultures (primary neursphsres (PNs) or secondary neurospheres (SNs) ) by fluorescence activated cell sorting and subjected to the gene expression microrray analysis.
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| Contributor(s) |
Tsuyama J, Shimazaki T, Okano H |
| Citation(s) |
26212661 |
| Submission date |
Jun 22, 2015 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Jun Tsuyama |
| E-mail(s) |
tsuyama.j@a7.keio.jp
|
| Organization name |
Keio University School of Medicine
|
| Street address |
35 Shinanomachi
|
| City |
Shinjuku-ku |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE70131 |
MicroRNA-153 regulates the acquisition of gliogenic competence by neural stem cells |
|
| Relations |
| BioProject |
PRJNA287695 |
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