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| Status |
Public on Jun 23, 2015 |
| Title |
Expression analysis of a full SlrV locus 1 knockout in Haemophilus influenzae strain PittII |
| Platform organism |
Haemophilus influenzae |
| Sample organism |
Haemophilus influenzae PittII |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of whole genome gene expression differences between a full (four-gene) SlrV locus 1 deletion compared to the WT PittII strain. Removal of the four SlrV genes (aka macrophage survival factors : Δmsf) results in a competetive defect with the WT strain in in vitro biofilms and in the otitis media chinchilla model. It also results in an inability to persist within macrophages long-term in vitro and to escape from the middle-ear space and infect other sites in the chinchilla model.
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| Overall design |
WT and Δmsf strains were grown in a chemically defined media (CDM) to early stationary phase (OD ~1.0). RNA was extracted from two separate cultures (biological replicates). 3-13 probes represent each subcluster(gene) and two replicate probe sets are included on each custom NTHi supragenome chip (technical replicates) thus under each sampling condition 4 separate values are obtained per strain and used for WT:Msf-KO comparisons.
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| Contributor(s) |
Kress-Bennett J, Hiller LN, Eutsey R, Powell E, Longwell M, Hillman T, Blackwell T, Byers B, Mell JC, Post CJ, Hu FZ, Ehrlich GD, Janto BA |
| Citation(s) |
26977929 |
| Submission date |
Jun 23, 2015 |
| Last update date |
Oct 08, 2019 |
| Contact name |
Benjamin Janto |
| E-mail(s) |
bjanto@drexelmed.edu
|
| Phone |
412-762-1903
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| Organization name |
Drexel University College of Medicine
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| Department |
Microbiology & Immunology
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| Street address |
245 N. 15th St
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19102 |
| Country |
USA |
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| Platforms (1) |
| GPL16197 |
NimbleGen Haemophilus influenzae supragenome 72K array v1.0 [090514_H_influenza_ASRI_RE_expr] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA287790 |
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