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| Status |
Public on Feb 06, 2019 |
| Title |
rDNA chromatin interactions in the MYC-driven Eμ-Myc transgenic mouse model of B-cell lymphoma [4C-seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Long-range interactions of ribososomal DNA (rDNA) identified by 4C-seq in mouse wildtype, premalignant and malignant Eμ-Myc cells and tissue culture adapated Eμ-Myc-shUbtf and Eμ-Myc-LMP (empty vector) cells.
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| Overall design |
We performed 4C-seq in mouse wildtype, premalignant and malignant cells primary preB Eμ-Myc cells and tissue culture adapated Eμ-Myc-shUbtf and Eμ-Myc-LMP. For this we used multiple bait primers across the rDNA and identified long-range interactions in 5000bp windows. The interaction frequency, represented as read counts per window, was compared between wt, prem and mal cells and only interactions also identified in Eμ-Myc-shUbtf and Eμ-Myc-LMP used for further analysis.
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| Contributor(s) |
Diesch J, Poortinga G |
| Citation(s) |
30701204 |
| Submission date |
Jun 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeannine Diesch |
| E-mail(s) |
jeannine.diesch@gmail.com
|
| Organization name |
Josep Carreras Leukaemia Research Institute
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| Department |
Research
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| Lab |
Chromatin, metabolism and cell fate
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| Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
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| City |
Badalona |
| State/province |
Barcelona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE70226 |
rDNA chromatin interactions in the MYC-driven Eµ-Myc transgenic mouse model of B-cell lymphoma |
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| Relations |
| BioProject |
PRJNA287915 |
| SRA |
SRP059832 |
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