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| Status |
Public on Jun 25, 2015 |
| Title |
Indirect Flight Muscle (IFM) expression profiling of actin and myosin nulls in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Expression profiling of IFMs from 1-2 day old adult male flies of 3 genotypes: Canton-S, IFM-specific actin null (Act88FKM88) and IFM-specific myosin null (Mhc7).
Results provide insight into how muscles respond to the absence of major scaffold proteins, and whether these transcriptional responses are filament-specific or generic to the tissue.
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| Overall design |
RNA was extracted from the IFMs of 1-2 day old adult male flies of 3 genotypes (Canton-S, Act88FKM88, Mhc7), labeled, and hybridized on Affymetrix GeneChip Drosophila Genome 2.0 microarrays. 9 microarray experiments from 3 independent biological replicates of each genotype. Data was analyzed using RMA method, and significance was tested using SAM version 3.02, with cut-off parameters of 10% FDR and minimum 2-fold change in expression levels.
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| Contributor(s) |
Madan A, Thimmaiya D, Franco-Cea A, Aiyaz M, Kumar P, Sparrow JC, Nongthomba U |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 24, 2015 |
| Last update date |
May 04, 2018 |
| Contact name |
Aditi Madan |
| E-mail(s) |
aditimdn@gmail.com
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| Organization name |
Indian Institute of Science
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| Department |
Dept. of MRDG
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| Lab |
Dr. Upendra's Lab, DBGL (Dev. & Biomed. Genet. Lab)
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| Street address |
GB-03,Dept. of MRDG,New BioSciences Bldg, IISc
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| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560012 |
| Country |
India |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA288041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE70252_RAW.tar |
27.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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