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| Status |
Public on Mar 31, 2007 |
| Title |
Expression profiling in monozygotic twins discordant for bipolar disorder |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify genes dysregulated in bipolar disorder (BD1) we carried out global gene expression profiling using whole-genome microarrays. To minimize genetic variation in gene expression levels between cases and controls we compared expression profiles in lymphoblastoid cell lines from monozygotic twin pairs discordant for the disease. We identified 82 genes that were differentially expressed by ≥ 1.3-fold in 3 BD1 cases compared to their co-twins, and which were statistically (p ≤ 0.05) differentially expressed between the groups of BD1 cases and controls. Using qRT-PCR we confirmed the differential expression of some of these genes, including: KCNK1, MAL, PFN2, TCF7, PGK1, and PI4KCB, in at least 2 of the twin pairs. In contrast to the findings of a previous study by Kakiuchi and colleagues with similar discordant BD1 twin design1 our data do not support the dysregulation of XBP1 and HSPA5. From pathway and gene ontology analysis we identified up-regulation of the WNT signalling pathway and the biological process of apoptosis. The differentially regulated genes and pathways identified in this study may provide insights into the biology of BD1.
Keywords: Bipolar Disorder, Monozygotic Twins, Discordant, Trasnsformed Lymphocytes
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| Overall design |
Three pairs of Monozygotic Twins which were discordant for Bipolar Disorder with psychotic features were examined. (n=6)
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| Contributor(s) |
Matigian N, Windus L, Smith H, Filippich C, Pantelis C, McGrath J, Mowry B, Hayward N |
| Citation(s) |
17440432 |
| Submission date |
Feb 14, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Nicholas Matigian |
| E-mail(s) |
n.matigian@griffith.edu.au
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| Phone |
61 7 38753660
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| Organization name |
National Adult Stem Cell Centre
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| Lab |
Systems Biology
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| Street address |
170 Kessels Rd, Nathan
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| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4111 |
| Country |
Australia |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM162897 |
Trasnsformed Lymphocytes Bipolar Disorder Affected Twin Pair 1 (DCBD1.1)(TP5) |
| GSM162898 |
Trasnsformed Lymphocytes Unaffected Twin Pair 1(DCBD1.2)(TP6) |
| GSM162899 |
Trasnsformed Lymphocytes Bipolar Disorder Affected Twin Pair 2 (DCBD2.1)(TP7) |
| GSM162900 |
Trasnsformed Lymphocytes Unaffected Twin Pair 2 (DCBD2.2)(TP8) |
| GSM162901 |
Trasnsformed Lymphocytes Bipolar Disorder Affected Twin Pair 3 (DCBD3.1)(TP9) |
| GSM162903 |
Trasnsformed Lymphocytes Unaffected Twin Pair 3 (DCBD3.2)(TP10) |
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| Relations |
| BioProject |
PRJNA98345 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7036_RAW.tar |
30.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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