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Series GSE7038 Query DataSets for GSE7038
Status Public on Jun 03, 2008
Title RLSC and MMH-D3 cell lines
Organism Mus musculus
Experiment type Expression profiling by array
Summary Increasing evidence provide support that the mammalian liver contains stem/progenitor cells, but their molecular phenotype, embryological derivation, cell biology as well as of their role in the liver cell turnover and regeneration remain to be further clarified.
We report the isolation, characterization and reproducible establishment in line of a resident liver stem cell (RLSC) with immunophenotype and differentiative potential distinct from other previously reported liver precursor/stem cells. RLSCs, derived from foetal and neonatal murine livers as well as from immortalized hepatocytic MMH lines and established in lines, are Sca+, CD34-, CD45-, alfa-fetoprotein+, albumin-. This immunophenotype suggests a non-haematopoietic origin. RLSCs transcriptional profile, defined by microArray technology, highlighted the expression of a broad spectrum of “plasticity-related genes” and “developmental genes” suggesting a multi-differentiative potential.
RLSCs, indeed, spontaneously differentiate into hepatocytes and cholangiocytes and, when cultured in appropriate conditions, into mesenchymal and neuro-ectodermal cell lineages, such as osteoblasts/osteocytes, chondrocytes, astrocytes and neuronal cells.
RLSCs capability to spontaneously differentiate into hepatocytes, the lack of albumin expression and the broad differentiative potentiality locate them in a pre-hepatoblast/liver precursor cells hierarchical position.
In conclusion, RLSCs may provide an useful tool to improve liver stem cell knowledge and to assess new therapeutic approaches for liver diseases.
Keywords: cell type comparison
 
Overall design We compared the gene expression profile of a RLSC line to that of the previously characterised hepatocytic differentiated cell line MMH-D3 (ref Amicone), using Affymetrix Mouse430_2 GeneChip array.
 
Contributor(s) Conigliaro A, Colletti M, Cicchini C, Guerra MT, Manfredini R, Zini R, Bordoni V, Siepi F, Leopizzi M, Tripodi M, Amicone L
Citation(s) 17932501
Submission date Feb 15, 2007
Last update date Feb 11, 2019
Contact name Rossella Manfredini
E-mail(s) manfredini.rossella@unimore.it
Phone +390592058065
Organization name Centre for Regenerative Medicine
Department Life Sciences
Street address Via Gottardi 100
City Modena
ZIP/Postal code 41100
Country Italy
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM162807 MMH-D3 cell line rep1
GSM162863 MetE14/3 RLSC cell line
GSM216535 WTE14/1 RLSC cell line
Relations
BioProject PRJNA98349

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7038_RAW.tar 40.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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