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| Status |
Public on Feb 06, 2008 |
| Title |
Transcriptome profile of Trypanosoma cruzi-infected cells |
| Platform organism |
Homo sapiens |
| Sample organisms |
Trypanosoma cruzi; Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
As Trypanosoma cruzi, the etiological agent of Chagas disease, multiplies in the cytoplasm of nucleated host cells, infection with this parasite is highly likely to affect host cells. We performed an exhaustive transcriptome analysis of T. cruzi-infected HeLa cells using an oligonucleotide microarray containing probes for greater than 47,000 human gene transcripts. In comparison with uninfected cells, those infected with T. cruzi showed greater than threefold up-regulation of 41 genes and greater than threefold down-regulation of 23 genes. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) of selected, differentially expressed genes confirmed the microarray data. Many of these up- and down-regulated genes were related to cellular proliferation, including seven up-regulated genes encoding proliferation inhibitors and three down-regulated genes encoding proliferation promoters, strongly suggesting that T. cruzi infection inhibits host cell proliferation, which may allow more time for T. cruzi to replicate and produce its intracellular nests. These findings provide new insight into the molecular mechanisms by which intracellular T. cruzi infection influences the host cell, leading to pathogenicity.
Keywords: infection response
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| Overall design |
Three replicates of infected and uninfected HeLa cell were analyzed. To examine the extent of cross hybridization between T. cruzi cRNA and Human chip, trypomastigote cRNA was hybridized with the same chip.
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| Contributor(s) |
Shigihara T, Hashimoto M, Shindo N, Aoki T |
| Citation(s) |
18058129 |
| Submission date |
Feb 16, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Takako Shigihara |
| E-mail(s) |
siggy@med.juntendo.ac.jp
|
| Organization name |
Juntendo University Graduate School of Medicine
|
| Department |
Biomedical Research Center
|
| Lab |
Division of Molecular and Biochemical Research
|
| Street address |
2-1-1, Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA98507 |
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