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Series GSE70554 Query DataSets for GSE70554
Status Public on Jul 20, 2015
Title Single-cell analysis reveals a stem cell program in human metastatic breast cancer cells (Human patients - mammary cells)
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary Despite major advances in understanding the molecular and genetic basis of cancer, metastasis remains the cause of >90% of cancer-related mortality1. Understanding metastasis initiation and progression is critical to develop new therapeutic strategies to treat and prevent metastatic disease. Prevailing theories hypothesize that metastases are seeded by rare tumor cells with unique properties, which may function like stem cells in their ability to initiate and propagate metastatic tumors.2 3-5 However, the identity of metastasis-initiating cells in human breast cancer remains elusive, and whether metastases are hierarchically organized is unknown.2 Here we show at the single-cell level that early stage metastatic cells possess a distinct stem-like gene expression signature. To identify and isolate metastatic cells from patient-derived xenograft (PDX) models of human breast cancer, we developed a highly sensitive FACS-based assay, which allowed us to enumerate metastatic cells in mouse peripheral tissues. We compared gene signatures in metastatic cells from tissues with low vs. high metastatic burden. Metastatic cells from low-burden tissues were distinct due to their increased expression of stem cell, EMT, pro-survival, and dormancy-associated genes. In contrast, metastatic cells from high-burden tissues were similar to primary tumor cells, which were more heterogeneous and expressed higher levels of luminal differentiation genes. Transplantation of stem-like metastatic cells from low-burden tissues showed that they have significant tumor-initiating capacity, and differentiate to produce luminal-like cancer cells. Progression to high metastatic burden was associated with increased proliferation and cMYC expression, which could be attenuated by treatment with cyclin dependent kinase (CDK) inhibitors. These findings support a hierarchical model for metastasis, where metastases are initiated by stem-like cells that proliferate and differentiate to produce advanced metastatic disease.
 
Overall design 271 samples
 
Contributor(s) Lawson DA, Bhakta NR, Kessenbrock K, Prummel K, Yu Y, Takai K, Zhou A, Eyob H, Balakrishnan S, Wang C, Yaswen P, Goga A, Werb Z
Citation(s) 26416748
Submission date Jul 06, 2015
Last update date Sep 30, 2015
Contact name Devon Lawson
E-mail(s) Devon.Lawson@ucsf.edu
Organization name UCSF
Department Anatomy
Lab Zena Werb
Street address 513 Parnassus Ave Hsw 1301
City San Francisco
State/province California
ZIP/Postal code 94143
Country USA
 
Platforms (1)
GPL20665 Biomark 96.96 dynamic array single-cell real-time qRT-PCR
Samples (271)
GSM1809961 3-12-2013, Individual #1 SAMPLE 1
GSM1809962 3-12-2013, Individual #1 SAMPLE 2
GSM1809963 3-12-2013, Individual #1 SAMPLE 3
This SubSeries is part of SuperSeries:
GSE70555 Single-cell analysis reveals a stem cell program in human metastatic breast cancer cells
Relations
BioProject PRJNA289006

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70554_non-normalized.txt.gz 97.8 Kb (ftp)(http) TXT
Processed data included within Sample table
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