|
| Status |
Public on Jul 15, 2015 |
| Title |
Cas9 gRNA engineering for selectable genome editing, activation and repression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq after Cas9-gRNA transfection with different length gRNAs
|
| |
|
| Overall design |
we performed PolyA Selection and RNA-Seq on cells transfected with dCas9-VPR and a gRNA of each length (20nt, 16nt, or 14nt) targeting ACTC1, MIAT, or HBG1/2
|
| |
|
| Contributor(s) |
Chavez A, Ter-Ovanesyan D |
| Citation(s) |
26344044 |
| Submission date |
Jul 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
George Chruch |
| E-mail(s) |
gchurch@genetics.med.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Street address |
77 Ave Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA289388 |
| SRA |
SRP060637 |
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