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| Status |
Public on Mar 16, 2016 |
| Title |
Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 1] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Diploidy is a fundamental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ cells that serve to insure a diploid genome upon fertilization. Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian species, but as of yet not from humans. Here we analyzed a large collection of human parthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and maintenance of human ES cell lines with a normal haploid karyotype. Haploid human ES cells exhibited typical pluripotent stem cell characteristics such as self-renewal capacity and a pluripotency-specific molecular signature. Although haploid human ES cells resembled their diploid counterparts, they also displayed distinct properties including differential regulation of X chromosome inactivation and genes involved in oxidative phosphorylation, alongside reduction in absolute gene expression levels and cell size. Intriguingly, we found that a haploid genome is compatible not only with the undifferentiated pluripotent state, but also with differentiated somatic fates representing all three embryonic germ layers, despite a persistent dosage imbalance between the autosomes and X chromosome. We expect that haploid human ES cells will provide novel means for studying human functional genomics, development and evolution.
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| Overall design |
RNA sequencing analysis was performed on a total of 15 samples, including haploid and diploid human parthenogenetic embryonic stem cells at different differentiation states and cell cycle phases.
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| Contributor(s) |
Sagi I, Egli D, Benvenisty N |
| Citation(s) |
26982723 |
| Submission date |
Jul 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Sagi |
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
| Street address |
Givat Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE71458 |
Derivation and differentiation of haploid human embryonic stem cells |
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| Relations |
| BioProject |
PRJNA291275 |
| SRA |
SRP061707 |
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