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Series GSE7149 Query DataSets for GSE7149
Status Public on Oct 01, 2007
Title A Microarray-based Analysis of Transcriptional Compartmentalization in the Alimentary Canal of Anopheles gambiae
Platform organisms Plasmodium falciparum; Anopheles gambiae
Sample organism Anopheles gambiae
Experiment type Expression profiling by array
Summary Although the basic anatomical sub-divisions of the larval mosquito gut were established several decades ago, information regarding their exact physiological roles is rather scarce. Several studies have reported differences between larval gut compartments in various morphological and physiological aspects. Unfortunately, the fragmentary and incomplete nature of this information makes it hard to establish clear links to the specific and/or unique physiological roles of each gut region.
In this study, we attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. We took advantage of a commercially-available microarray platform containing ~14,000 different An. gambiae transcripts (Affymetrix GeneChip® Plasmodium/Anopheles array, Affymetrix Inc., Santa Clara, CA). Based on the information obtained, we discuss the putative physiological roles of each major compartment of the larval gut, and identify potential new targets for larval control strategies.
Keywords: tissue comparison
 
Overall design We attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. Signal intensities of transcripts from each gut section were compared to those of a matching group of whole 4th instar larvae using t-tests. Transcripts showing an intensity value two-fold or higher (p < 0.05) in each gut section as compared to whole larvae were considered to be significantly enriched.
 
Contributor(s) Neira Oviedo M, VanEkeris L, Corena-Mcleod M, Linser P
Citation(s) 18237285
Submission date Feb 27, 2007
Last update date Jan 24, 2013
Contact name Marco V. Neira
E-mail(s) mneira@whitney.ufl.edu
Phone 904-461-4037
Fax 904-461-4052
Organization name University of Florida
Department The Whitney Laboratory
Lab Mosquito group/Paul Linser's Lab.
Street address 9505 Oceanshore Blvd.
City St. Augustine
State/province FL
ZIP/Postal code 32080
Country USA
 
Platforms (1)
GPL1321 [Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array
Samples (15)
GSM172187 larval_gut_gastric_caeca_rep1
GSM172188 larval_gut_gastric_caeca_rep2
GSM172189 larval_gut_gastric_caeca_rep3
Relations
BioProject PRJNA98193

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Supplementary file Size Download File type/resource
GSE7149_RAW.tar 29.7 Mb (http)(custom) TAR (of CEL)

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