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| Status |
Public on Oct 02, 2016 |
| Title |
Liver-resident memory CD8+ T cells form a front-line defense against malaria liver-stage infection. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In recent years, various intervention strategies have reduced malaria morbidity and mortality, but further improvements likely depend upon development of a broadly protective vaccine. To better understand immune requirement for protection, we examined liver-stage immunity after vaccination with irradiated sporozoites, an effective though logistically difficult vaccine. We identified a population of memory CD8+ T cells that expressed the gene signature of tissue-resident memory (Trm) T cells and remained permanently within the liver, where they patrolled the sinusoids. Exploring the requirements for liver Trm cell induction, we showed that by combining dendritic cell-targeted priming with liver inflammation and antigen recognition on hepatocytes, high frequencies of Trm cells could be induced and these cells were essential for protection against malaria sporozoite challenge. Our study highlights the immune potential of liver Trm cells and provides approaches for their selective transfer, expansion or depletion, which may be harnessed to control liver infections or autoimmunity.
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| Overall design |
9 samples were analyzed: 3 replicates of memory CD69+KLRG1low PbT-I CD8+ T cells and 3 replicates of memory CD69-KLRG1+ PbT-I CD8+ T cells isolated from the liver of C57/BL6 mice on day 30 p.i. with irradiated sporozoites; 3 replicates of memory KLRG1+CD69- PbT-I CD8+ T cells (Tem) isolated from the spleen of the same C57/BL6 mice on day 30 p.i. with irradiated sporozoites.
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| Contributor(s) |
Fernandez-Ruiz D, Ng WY, Holz L, Ma JZ, Zaid A, Wong YC, Lau LS, Mollard V, Cozijnsen A, Collins N, Li J, Davey GM, Kato Y, Devi S, Skandari R, Pauley M, Manton JH, Godfrey DI, Braun A, Tay SS, Tan PS, Bowen DG, Koch-Nolte F, Rissiek B, Carbone FR, Crabb BS, Lahoud MH, Cockburn I, Mueller SN, Bertolino P, McFadden GI, Caminschi I, Heath WR |
| Citation(s) |
27692609 |
| Submission date |
Jul 29, 2015 |
| Last update date |
Jun 02, 2017 |
| Contact name |
Joel Zhi-Iong Ma |
| E-mail(s) |
intuitivelogic.au@gmail.com
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| Organization name |
University of Melbourne
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| Street address |
Grattan Street
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| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3010 |
| Country |
Australia |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA291427 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71518_RAW.tar |
36.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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