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Series GSE7155 Query DataSets for GSE7155
Status Public on Jul 30, 2007
Title Expression data from adult laboratory mouse brain hemispheres
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inbred congenic strain B6.C6.132.54/Vad was created using C57BL/6ByJ background and BALB/cJ donor strains. Flanking background markers at chr. 6: 75.9 Mb (rs4226008, NCBI Mouse Build 36 / dbSNP Build 126) and 122.3 Mb (rs3023093), and limiting donor markers at 81.9 Mb (rs4226024) and at 91.8 Mb (rs3712161) defined the introgressed region. We concluded the segment size must be between 9.9 Mb and 46.4 Mb. In a Quantitative Trait Gene identification study we compared brain (without cerebellum) gene expression between progenitors and congenics. Such comparisons can facilitate identification of cis-regulated genes and to establish genetic control of a complex phenotype whose expression is associated with the introgressed chromosome segment.
We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process.
Keywords: strain comparison
Overall design Experiments were carried out in three batches. In each batch 10 animals per strain were used. After processing each hemisphere separately, 10 hemispheres were pooled for one high-density oligonucleotide microarray (Mouse Genome 430 2.0, Affymetrix, Santa Clara, CA). For each strain 3 oligonucleotide microarrays were used (n=3). We used adult, untreated, male mice (n=30, 90 mice in toto).
Contributor(s) Vadasz C, Saito M
Citation(s) 17936574
Submission date Feb 28, 2007
Last update date Feb 11, 2019
Contact name Csaba Vadasz
Organization name New York University NKI
Department Center for Neurobehavior Genetics
Street address 140 Old Orangeburg Road
City Orangeburg
State/province NY
ZIP/Postal code 10962
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM172395 C57BL/6ByJ strain, brain hemisphere, biological rep1
GSM172396 C57BL/6ByJ strain, brain hemisphere, biological rep2
GSM172397 C57BL/6ByJ strain, brain hemisphere, biological rep3
BioProject PRJNA98273

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Supplementary file Size Download File type/resource
GSE7155_RAW.tar 28.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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