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| Status |
Public on Aug 04, 2015 |
| Title |
Molecular Mechanisms Associated with Primary Open-angle Glaucoma with MicroRNA Microarray Data |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Cytomegalovirus; Lymphocryptovirus; Rhadinovirus; JC polyomavirus; Human immunodeficiency virus 1; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
This study aimed to investigate the molecular mechanism responsible for primary open-angle glaucoma (POAG) progression. We analyzed microRNAs (miRNAs) expression profiling in aqueous humor (AH) of both POAG patients and normal controls, using a microarray-based approach. Subsequently, differentially expressed miRNAs (DEmiRNAs) were identified using Bayes moderated t-test. Next, DEmiRNAs target genes were predicted based on miRNA databases, followed by GO analysis and pathway analysis using DAVID. Furthermore, OAG-related genes analysis for target genes was carried out using CTD database, respectively. Finally, verification of DEmiRNAs expression levels was performed by RT-qPCR. A total of 40 significant DEmiRNAs were identified between control and POAG groups, including 24 up-regulated miRNAs and 16 down-regulated miRNAs. Further, the target genes of hsa-miR-206, including BMP2, SMAD4, ID2, and TNF, were mainly enriched in transforming growth factor-β (TGF-β) signaling pathway. While, target genes of hsa-miR-184, hsa-miR-34c-5p, hsa-miR-7-2-3p and hsa-miR-20b-3p, including BCL2, EPHB2, VEGFA, COL4A1, APC, and TGFBR1, were enriched in eye development. Moreover, FNDC3B, CAV2 and VEGF, target genes of hsa-miR-206 or hsa-miR-34c-5p, were the OAG-related genes. Ultimately, RT-qPCR analysis confirmed that mRNA levels of hsa-miR-206, hsa-miR-7-2-3p, and hsa-miR-20b-3p were increased, while those of hsa-miR-184 and hsa-miR-34c-5p were decreased in POAG compared with normal groups (P < 0.05). Hsa-miR-206, hsa-miR-184, hsa-miR-34c-5p, hsa-miR-7-2-3p and hsa-miR-20b-3p might play a significant role in the pathogenesis of POAG and hsa-miR-206 might be associated with the development of POAG by regulating TGF-β signaling pathway. These results might provide insight toward a better understanding of the pathogenesis of POAG.
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| Overall design |
Fifteen patients with ocular hypertensive POAG, who needed glaucoma filtrating surgeries, were enrolled in this studydisease group:PA group,PB group,PC group;each group of five patients). Fifteen patients with cataract undergoing cataract surgery were recruited as normal group (normal group:NA group,NB group,NC group;each group of five patients).
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| Contributor(s) |
Xu H, Kong X, Wang J, Sun X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 03, 2015 |
| Last update date |
Aug 06, 2015 |
| Contact name |
Xiangmei Kong |
| Phone |
+86-021-64377134
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| Organization name |
Eye & ENT Hospital of Fudan University
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| Department |
Department of Ophthalmology
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| Street address |
83 Fenyang Road, Xuhui District
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| City |
Shanghai，China |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL19128 |
Exiqon miRCURY LNA microRNA array; 7th generation - hsa, mmu & rno (miRBase 18.0) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA291691 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71639_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE71639_normalized_data_matrix.txt.gz |
719 b |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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