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| Status |
Public on Sep 29, 2015 |
| Title |
Gene expression data from mouse squamous cell carcinoma cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We describe a function of focal adhesion kinase (FAK) in driving anti-tumor immune evasion. The kinase activity of nuclear-targeted FAK in squamous cancer cells drives exhaustion of CD8+ T-cells and recruitment of regulatory T-cells by transcriptionally regulating chemokine/cytokine and ligand-receptor networks, including transcription of Ccl5 that is crucial. These changes inhibit antigen-primed cytotoxic CD8+ T-cell activity, permitting growth of FAK-expressing tumors.
To address how FAK activity in squamous cell carcinoma (SCC) cancer cells promotes elevated intra-tumoral regulatory T-cells, we analyzed global transcriptional profiles of SCC cells expressing or not expressing FAK.
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| Overall design |
Total RNA was isolated from three biological replicates each of SCC cells from mice genetically null for Fak (SCC FAK-/-) and cells stably re-expressing FAK (SCC FAK-wt) and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Serrels A, Lund T, Serrels B, Byron A, Sims AH, Frame MC |
| Citation(s) |
26406376 |
| Submission date |
Aug 03, 2015 |
| Last update date |
Sep 29, 2015 |
| Contact name |
Adam Byron |
| Organization name |
University of Edinburgh
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| Department |
Edinburgh Cancer Research UK Centre
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| Street address |
Western General Hospital, Crewe Road South
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| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XR |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20766 |
[Mouse4302_Mm_ENSG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mouse4302_Mm_ENSG_17.0.0] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA291759 |
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