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Series GSE71662 Query DataSets for GSE71662
Status Public on Sep 29, 2015
Title Gene expression data from mouse squamous cell carcinoma cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We describe a function of focal adhesion kinase (FAK) in driving anti-tumor immune evasion. The kinase activity of nuclear-targeted FAK in squamous cancer cells drives exhaustion of CD8+ T-cells and recruitment of regulatory T-cells by transcriptionally regulating chemokine/cytokine and ligand-receptor networks, including transcription of Ccl5 that is crucial. These changes inhibit antigen-primed cytotoxic CD8+ T-cell activity, permitting growth of FAK-expressing tumors.
To address how FAK activity in squamous cell carcinoma (SCC) cancer cells promotes elevated intra-tumoral regulatory T-cells, we analyzed global transcriptional profiles of SCC cells expressing or not expressing FAK.
 
Overall design Total RNA was isolated from three biological replicates each of SCC cells from mice genetically null for Fak (SCC FAK-/-) and cells stably re-expressing FAK (SCC FAK-wt) and hybridized on Affymetrix microarrays.
 
Contributor(s) Serrels A, Lund T, Serrels B, Byron A, Sims AH, Frame MC
Citation(s) 26406376
Submission date Aug 03, 2015
Last update date Sep 29, 2015
Contact name Adam Byron
Organization name University of Edinburgh
Department Edinburgh Cancer Research UK Centre
Street address Western General Hospital, Crewe Road South
City Edinburgh
ZIP/Postal code EH4 2XR
Country United Kingdom
 
Platforms (1)
GPL20766 [Mouse4302_Mm_ENSG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mouse4302_Mm_ENSG_17.0.0]
Samples (6)
GSM1842579 SCC-FAK-wt_rep1
GSM1842580 SCC-FAK-wt_rep2
GSM1842581 SCC-FAK-wt_rep3
Relations
BioProject PRJNA291759

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Supplementary file Size Download File type/resource
GSE71662_RAW.tar 34.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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