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| Status |
Public on Aug 31, 2015 |
| Title |
Expression data from CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mice, compared to controls |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Myeloid derived suppressor cells (MDSCs) are an immunosuppressive population of immature myeloid cells found in advanced stage cancer patients and mouse tumor models. To identify potential genes playing essential role in MDSC biology, we have conducted microarray analysis of gene expression in MDSCs from esophageal tumor-bearing mice, compared to immature myeloid cells from healthy littermate control mice.
In this dataset, we include the expression data obtained from sorted splenic CD11b+Gr1+ cells from tumor-bearing L2-Cre;p120f/f mice, compared to healthy littermate controls. Using these data, we have identified 964 genes showing differential expression between the two groups. Among these was the Cd38 gene, which was among the genes most upregulated in MDSCs from tumor-bearing mice.
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| Overall design |
9 total samples were analyzed: 6 sample from experimental tumor-bearing mice and three pooled samples from control mice. Gene expression difference was determined by univariate test (two-sample t-test) with multivariate permutation test (10,000 random permutations). A cut-off p-value of less than 0.001 and minimum 2-fold expression change were used to identify genes with significant expression differences between the two groups.
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| Contributor(s) |
Waldron TJ, Rustgi AK |
| Citation(s) |
26294209 |
| Submission date |
Aug 04, 2015 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Anil K. Rustgi |
| Organization name |
University of Pennsylvania
|
| Department |
Gastroenterology
|
| Lab |
Rustgi Lab
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| Street address |
421 Curie Boulevard
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM1843429 |
CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mouse, biological rep1 |
| GSM1843430 |
CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mouse, biological rep2 |
| GSM1843431 |
CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mouse, biological rep3 |
| GSM1843432 |
CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mouse, biological rep4 |
| GSM1843433 |
CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mouse, biological rep5 |
| GSM1843434 |
CD11b+Gr1+ splenocytes from tumor-bearing L2-Cre;p120f/f mouse, biological rep6 |
| GSM1843435 |
Pooled CD11b+Gr1+ splenocytes from 3 healthy L2-Cre;p120+/f mice, biological rep1 |
| GSM1843436 |
Pooled CD11b+Gr1+ splenocytes from 3 healthy L2-Cre;p120+/f mice, biological rep2 |
| GSM1843437 |
Pooled CD11b+Gr1+ splenocytes from 3 healthy L2-Cre;p120+/f mice, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE71707 |
Myeloid derived suppressor cells from tumor-bearing L2-Cre;p120f/f mice |
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| Relations |
| BioProject |
PRJNA291867 |
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