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| Status |
Public on Nov 02, 2007 |
| Title |
Differential gene expression analysis of iodide treated rat thyroid follicular cell line PCCl3 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by SAGE
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| Summary |
The inhibitory effect of supra physiological iodide concentrations on thyroid hormone synthesis (Wolff-Chaikoff effect) and thyrocyte proliferation is largely know as iodine autoregulation. However, the molecular mechanisms by which iodide excess modulate thyroid functions remains unclear. In this work, we analyzed the rat follicular cell PCCl3 transcriptome profile under untreated and treated conditions with 10-3M sodium iodide (Na/I). Serial Analysis of Gene Expression revealed several transcripts differentially expressed in response to the iodide showing that excess iodide affects almost all aspects of thyroid cell function and differentiation acting on the iodine autoregulatory mechanism through a complex process.
Keywords: comparative genomic analysis
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| Overall design |
PCCl3 cells were cultured under untreated and treated conditions with 10-3M NaI during 24 hours
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| Contributor(s) |
Leoni SG, Galante PA, Ricarte JM, Kimura ET |
| Citation(s) |
18272324 |
| Submission date |
Mar 02, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Edna Teruko Kimura |
| E-mail(s) |
etkimura@usp.br
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| Phone |
55-11-30917304
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| Fax |
55-11-30917402
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| Organization name |
USP - University of Sao Paulo
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| Department |
ICB - Cell and Developmental Biology
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| Lab |
Thyroid Molecular Biology
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| Street address |
Av Prof Lineu Prestes 1524 sala 414
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| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
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| Platforms (1) |
| GPL23 |
SAGE:10:NlaIII:Rattus norvegicus |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA98247 |
