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Series GSE71774 Query DataSets for GSE71774
Status Public on Aug 07, 2015
Title Remodeling of Brown and White Adipose Tissue by NT-PGC-1α-Mediated Gene Regulation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The β-adrenergic receptor signaling pathway is a major component of adaptive thermogenesis in brown and white adipose tissue during cold acclimation. The β-AR activation highly induces transcriptional coactivator PGC-1α and its splice variant N-terminal (NT)-PGC-1α, promoting the transcription program of mitochondrial biogenesis and thermogenesis. In the present study, we evaluated the role of NT-PGC-1α in brown adipocyte energy metabolism by genome-wide profiling of NT-PGC-1α-responsive genes. Canonical pathway analysis revealed that a number of genes upregulated by NT-PGC-1α are highly enriched in mitochondrial pathways including fatty acid transport and β-oxidation, TCA cycle and electron transport system, thus reinforcing the crucial role of NT-PGC-1α in the enhancement of mitochondrial function. Moreover, gene expression profiling of NT-PGC-1α revealed activation of distinct metabolic pathways such as glucose, lipid and nucleotide metabolism and of signaling pathways such as RAR and PPAR-γ/RXRα activation in brown adipocytes. Together, our data strengthen our previous findings that NT-PGC-1α is a key regulator of mitochondrial oxidative metabolism and thermogenesis in brown adipocytes and further suggest that NT-PGC-1α influences a broader spectrum of thermogenic processes to meet cellular needs for adaptive thermogenesis.
 
Overall design Two samples from two groups: NT-PGC-1α overexpression and empty vector. There are technical replicates (A and B) for each group. Two RNA samples were pooled for each group.
 
Contributor(s) Kim J, Fernand VE, Henagan T, Shin J, Huypens P, Newman S, Gettys TW, Chnag JS
Citation(s) 27454177
Submission date Aug 06, 2015
Last update date Aug 31, 2016
Contact name Ji Suk Chang
Organization name Pennington Biomedical Research Center
Department Gene Regulation and Metabolism
Street address 6400 Perkins Rd.
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
 
Platforms (1)
GPL2995 ABI Mouse Genome Survey Microarray
Samples (4)
GSM1845084 empty vector [1A]
GSM1845085 empty vector [1B]
GSM1845086 NT-PGC-1α [4A]
Relations
BioProject PRJNA292062

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71774_non-normalized.txt.gz 680.0 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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