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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 10, 2016 |
| Title |
Gene expression data from mouse regulatory T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Intravenous Immunoglobulin (IVIg) is widely used as an immunomodulatory therapy. We have recently demonstrated that IVIg protects against airway hyper-reactivity (AHR) and inflammation in mouse models of allergic airway disease (AAD), associated with induction of Foxp3+ regulatory T cells (Treg). Using DEREG (DEpletion of REGulatory T cell) mice, in which endogenous Treg can be ablated with Diphtheria toxin (DTx) treatment, we demonstrate that IVIg generates a de novo population of induced Treg (iTreg) in the absence of endogenous Treg. IVIg-generated iTreg were sufficient for inhibition of ovalbumin-induced AHR in an antigen-driven murine model of AAD. In the absence of endogenous Treg, IVIg failed to confer protection against AHR and airway inflammation. Adoptive transfer of purified IVIg-generated iTreg prior to antigen challenge effectively prevented airway inflammation and AHR in an antigen-specific manner.
The goal of this study was to characterize the gene expression profile of a pure population of IVIg-generated induced Treg (iTreg).
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| Overall design |
Treg were isolated from mice sensitized and challenged with ovalbumin (OVA). Animals were treated with IVIg to generate iTreg with or without DTx endogenous Treg pre-depletion. Human serum albumin (HSA) was used as a control protein treatment.
RNA was isolated from 4 biological replicates for each condition. 12 samples in total were hybridized to Affymetrix gene expression microarrays.
variable: treatment: OVA-HSA-OVA - DTx: BMR133, BMR134, BMR135, BMR136
variable: treatment: OVA-IVIg-OVA - DTx: BMR137, BMR138, BR139, BMR140
variable: treatment: OVA-IVIg-OVA + DTx: BMR141, BMR142, BMR143, BMR144
repeat: biological replicate: eTreg: BMR133, BMR134, BMR135, BMR136
repeat: biological replicate: e_iTreg: BMR137, BMR138, BR139, BMR140
repeat: biological replicate: IVIg-iTreg: BMR141, BMR142, BMR143, BMR144
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| Contributor(s) |
Kaufman GN, Mazer BD |
| Citation(s) |
28219891 |
| Submission date |
Aug 06, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Bruce David Mazer |
| E-mail(s) |
bruce.mazer@mcgill.ca
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| Organization name |
RI-MUHC
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| Department |
RESP
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| Street address |
1001 boul. Décarie
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H4A 3J1 |
| Country |
Canada |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA292115 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71811_RAW.tar |
112.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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