|
| Status |
Public on Jun 13, 2016 |
| Title |
FUS KO mRNA sequencing and anti-FUS RNA immunoprecipitation sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
The aim of our study is to identify the role of FUS in shaping the transcriptome. RNA-seq of two FUS KO clones was performed and compared to wt; for each, four replicates were sequenced.
RNA molecules associated with the FUS protein were determined by means of a RNA immuno-precipitation, followed by high-throughput sequencing. Total RNA was used as a control.
SH-SY5Y cells were used for both experiments.
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| |
|
| Overall design |
RNA-seq: 4 wt samples, 4 A4 KO samples, 4 A5 KO samples.
RIP-seq: 1 input control sample, 3 anti-FUS IP replicates.
|
| |
|
| Contributor(s) |
Ruepp MD, Muehlemann O, Colombo M |
| Citation(s) |
27252488 |
| Submission date |
Aug 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Oliver Muehlemann |
| Organization name |
University of Bern
|
| Street address |
Freiestrasse 6
|
| City |
Bern |
| ZIP/Postal code |
3012 |
| Country |
Switzerland |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA292114 |
| SRA |
SRP062130 |
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