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| Status |
Public on Aug 13, 2015 |
| Title |
Mouse lung tissue transcriptome response to influenza H5N1 and mutants [mRNA](Agilent-028005) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse lung infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203') and VN1203-NS1trunc124.
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| Overall design |
Groups of 22-week-old C57BL/6 mice were infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203') and VN1203-NS1trunc124 (an NS1 protein with a 90 amino acid truncation at the C-terminus). Infections were done at 10^4 PFU/mouse in 50 μl PBS. Time points were 1, 2, 4, and 7 d.p.i. There were 5 mice for all infection groups and 3 mice for all mock groups. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.
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| Contributor(s) |
Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y |
| Citation(s) |
34051754 |
| Submission date |
Aug 12, 2015 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
|
| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (37)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA292712 |
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