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Series GSE7211

Query DataSets for GSE7211

Status

Public on Mar 07, 2007

Title

A polyadenylation factor subunit implicated in regulating oxidative stress responses in Arabidopsis thaliana

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype.
We used microarrays to understand the link between the polyadenylation defect and stress tolerance.
Keywords: mutant, wild-type, complemented line comparison

Overall design

Arabidopsis plants (the wild-type, oxt6 mutant, and two independent oxt6 transformants each carrying a gene encoding just the smaller of the two At1g30460-encoded proteins) were seeded and grown in soil. Plants were harvested for RNA isolation after 30 days; for this, six samples derived from 10-15 plants were prepared for each line. Ten samples were selected for study – three wild-type, three oxt6, and two from each of the complemented lines. Analysis of independent complemented plants minimizes the possible effects that may arise due to T-DNA insertion.

Contributor(s)

Zhang J, Addepalli B, Yun K, Xu R, Li QQ, Rao S, Hunt AG, Falcone DF

Citation(s)

18545667

Submission date

Mar 06, 2007

Last update date

Dec 07, 2018

Contact name

Arthur G Hunt

E-mail(s)

aghunt00@uky.edu