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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 04, 2016 |
| Title |
Effect of deletion of carnitine palmitoyltransferase2 on interscapular BAT gene expression upon adrenergic stimulation in thermoneutral acclimatized mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Ambient temperature affects energy intake and expenditure to maintain homeostasis in a continuously fluctuating environment. Here, mice with an adipose-specific defect in fatty acid oxidation (Cpt2A-/-) were subjected to varying temperature to determine the role of adipose bioenergetics to environmental adaptation. Cpt2A-/- brown adipose tissue (BAT) failed to induce thermogenic genes such as Ucp1 and Pgc1α in response to adrenergic stimulation, which is exacerbated by increasing temperature. Thermoneutrality induced a mitochondrial DNA stress in Cpt2A-/- BAT that resulted in a loss of classical interscapular BAT, but did not affect body weight gain or glucose tolerance in response to a high-fat diet.
In this dataset, we include the expression data obtained from dissected mouse interscapular brown adipose tissue from mice acclimatized to thermoneutrality (30C) with and without beta3adrenergic stimulation with and without the deletion of carnitine palmitoyltransferase 2 (i.e., adipose unable to beta-oxidize long chain fatty acids in mitochondria).
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| Overall design |
WildType and Cpt2A KnockOut mice were treated either with or without beta3adrenergic stimulation, thus four classes. Three biologic replicates were compared per class, thus twelve mice.
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| Contributor(s) |
Wolfgang MJ, Lee J |
| Citation(s) |
26854223 |
| Submission date |
Aug 19, 2015 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Michael J. Wolfgang |
| E-mail(s) |
mwolfga1@jhmi.edu
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| Organization name |
The Johns Hopkins University School of Medicine
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| Department |
Institute for Basic Biomedical Sciences
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| Lab |
Rangos Bldg 475
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| Street address |
855 N Wolfe Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1857627 |
WT Interscapular Brown Adipose Tissue, injected with saline 3hrs prior to dissection, biological rep1 |
| GSM1857628 |
WT Interscapular Brown Adipose Tissue, injected with saline 3hrs prior to dissection, biological rep2 |
| GSM1857629 |
WT Interscapular Brown Adipose Tissue, injected with saline 3hrs prior to dissection, biological rep3 |
| GSM1857630 |
WT Interscapular Brown Adipose Tissue, injected with 10mg/kg CL316243 3hrs prior to dissection, biological rep1 |
| GSM1857631 |
WT Interscapular Brown Adipose Tissue, injected with 10mg/kg CL316243 3hrs prior to dissection, biological rep2 |
| GSM1857632 |
WT Interscapular Brown Adipose Tissue, injected with 10mg/kg CL316243 3hrs prior to dissection, biological rep3 |
| GSM1857633 |
Cpt2KO Interscapular Brown Adipose Tissue, injected with saline 3hrs prior to dissection, biological rep1 |
| GSM1857634 |
Cpt2KO Interscapular Brown Adipose Tissue, injected with saline 3hrs prior to dissection, biological rep2 |
| GSM1857635 |
Cpt2KO Interscapular Brown Adipose Tissue, injected with saline 3hrs prior to dissection, biological rep3 |
| GSM1857636 |
Cpt2KO Interscapular Brown Adipose Tissue, injected with 10mg/kg CL316243 3hrs prior to dissection, biological rep1 |
| GSM1857637 |
Cpt2KO Interscapular Brown Adipose Tissue, injected with 10mg/kg CL316243 3hrs prior to dissection, biological rep2 |
| GSM1857638 |
Cpt2KO Interscapular Brown Adipose Tissue, injected with 10mg/kg CL316243 3hrs prior to dissection, biological rep3 |
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| Relations |
| BioProject |
PRJNA293363 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72210_RAW.tar |
281.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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