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| Status |
Public on Feb 18, 2016 |
| Title |
Identifying molecular mechanisms of neural crest (NC) EMT and cell migration |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To identify differntially expressed transcripts in TP-0903 treated embryos that impair cranila NC EMT and cell migration in zebrafish embryos
Methods: zebrafish embryos treated at 13 hpf with 5-7uM TP-0903 and DMSO for 1-, 4- and 8-hrs at 28°C. 35 embryos were collected for each treatment.
Results: TP-0903 increases expression of several retinoic acid target genes including genes from within the retinoid pathway
Conclusions: TP-0903 causes a direct increase in RA signaling that impairs cranial NC EMT and cell migration in zebrafihs embryos
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| |
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| Overall design |
mRNA profiles of zebrafish embryos treated with TP-0903 and DMSO were generated by RNA-Seq, in quadruplicates, using Illumina Hi Seq
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| |
|
| Contributor(s) |
Jimenez L, Stewart R |
| Citation(s) |
26794130 |
| Submission date |
Aug 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Rodeny Stewart |
| E-mail(s) |
rodney.stewart@hci.utah.edu
|
| Organization name |
University of Utah
|
| Department |
Oncological Sciences
|
| Street address |
2000 Circle of Hope Dr
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (24)
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| Relations |
| BioProject |
PRJNA293748 |
| SRA |
SRP062788 |
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