|
| Status |
Public on Dec 03, 2015 |
| Title |
Identification of a portal vessel-associated fetal liver hematopoietic stem cell niche |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Whereas the cellular basis of the hematopoietic stem cell (HSC) niche in the bone marrow has been characterized, the nature of the fetal liver (FL) niche is not yet elucidated. We show that Nestin+NG2+ pericytes associate with portal vessels, forming a niche promoting HSC expansion. Nestin+NG2+ cells and HSCs scale during development with the fractal branching patterns of portal vessels, tributaries of the umbilical vein. After closure of the umbilical inlet at birth, portal vessels undergo a transition from Neuropilin-1+Ephrin-B2+ artery to EphB4+ vein phenotype, associated with a loss of peri-portal Nestin+NG2+ cells and emigration of HSCs away from portal vessels. These data support a model in which HSCs are titrated against a peri-portal vascular niche with a fractal-like organization enabled by placental circulation.
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| |
|
| Overall design |
Characterization of the transcriptome of fetal liver and adult bone marrow niche using RNA-seq
|
| |
|
| Contributor(s) |
Khan JA, Frenette P |
| Citation(s) |
26634440 |
| Submission date |
Aug 25, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jalal Ahmed Khan |
| E-mail(s) |
ahmed.ahmed@mssm.edu
|
| Organization name |
Icahn School of Medicine
|
| Department |
1Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research
|
| Lab |
Paul Frenett
|
| Street address |
1301 Morris Park Ave
|
| City |
Bronx |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA293804 |
| SRA |
SRP062819 |
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