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| Status |
Public on Dec 01, 2016 |
| Title |
Transcriptome profiling of madin-darby canine kidney cells with canine parvovirus 2a persistent infection |
| Platform organism |
Canis lupus familiaris |
| Sample organism |
Canis lupus |
| Experiment type |
Expression profiling by array
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| Summary |
It has been demonstrated that parvovirus infection causing cytotoxicity and immune activation effect in many virus-induced cell lines, however, the underlying mechanisms are not fully understood. To investigate the gene expression signature of host cell after CPV-2 infection, we performed transcriptome profiling of MDCK after persistent infection of CPV-2a using microarray analysis. Uninfected MDCK cells act as negative control. The data show the genes regulated by CPV-2 infection..
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| Overall design |
The monolayer MDCK cells were digested with trypsin and plated into new cell culture flasks, supplement with 1/10 (v/v) CPV-2 virus solution (MOI=1) in DMEM containing 5% FBS. The infected MDCK cells were cultured at 37°C with 5% CO2, and passage for ten generation. MDCK F10 cells infected with CPV-2a and mock-treatment were harvest for microarray analysis.
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| Contributor(s) |
Shu L, Sun S, Guo H |
| Citation(s) |
27339228 |
| Submission date |
Aug 26, 2015 |
| Last update date |
Mar 07, 2017 |
| Contact name |
Y Gao |
| E-mail(s) |
yyshpy@gmail.com
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| Organization name |
LVRI
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| Street address |
Xujiaping 1
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| City |
Lanzhou |
| ZIP/Postal code |
730046 |
| Country |
China |
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| Platforms (1) |
| GPL3738 |
[Canine_2] Affymetrix Canine Genome 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA293948 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72397_RAW.tar |
6.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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