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| Status |
Public on Nov 13, 2016 |
| Title |
AhR links air pollution and atopic dermatitis via Artemin induction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Atopic dermatitis is increasing worldwide, correlating with air pollutions. Various organic components of pollutants activate transcription factor AhR (aryl-hydrocarbon receptor). We have established AhR-CA mice, whose keratinocytes express constitutive-active AhR, and these mice developed atopic dermatitis-like frequent scratching and allergic inflammation. In this study we performed ChIP-seq analyses and identified keratinocyte-specific AhR target genes, including inflammatory cytokines Tslp and IL33, and neurotrophic factor Artemin. While AhR-CA mice exhibited epidermal hyperinnervation and alloknesis leading to hypersensitivity to pruritus, blockade of Artemin alleviated these phenotypes. AhR-CA mice showed scratching-induced barrier insufficiency and enhanced sensitization to epicutaneously-applied antigens, recapitulating human atopic dermatitis. Consistently, AhR activation and Artemin expression was detected in the epidermis of atopic dermatitis patients and keratinocytes exposed to air pollutants. Thus, AhR in keratinocytes senses the environmental stimuli and responds to them through moderating inflammation. We propose a mechanism in which air pollution induces atopic dermatitis through AhR activation.
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| Overall design |
Gene expression in epidermis obtained from wild-type and AhRCA neonates. In AhRCA mice, constitutive active form of AhR transcription factor is expressed under the control of keratin 14 promoter. Three independent samplings were performed, and each sampling includes RNA from one AhRCA mice and one littermate wild-type mice, respectively.
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| Contributor(s) |
Hidaka T, Ogawa E, H. Kobayashi E, Suzuki T, Funayama R, Nagashima T, Fujimura T, Aiba S, Nakayama K, Okuyama R, Yamamoto M |
| Citation(s) |
27869817 |
| Submission date |
Aug 27, 2015 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Eri H Kobayashi |
| Organization name |
Tohoku University Graduate School of Medicine
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| Department |
Medical Biochemistry
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| Street address |
2-1 Seiryo-machi, Aobaku
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| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA294081 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72449_RAW.tar |
13.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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