 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 16, 2015 |
| Title |
Comparison of gene expression by sheep and human blood stimulated with the TLR4 agonists lipopolysaccharide and monophosphoryl lipid A [human] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
11,431 and 4,992 genes were determined in whole blood of healthy human volunteers and normal sheep, respectively following MPLA and LPS exposure
Following the exposure 1,029 human and 175 sheep genes were differentially expressed. Of those 175 sheep genes, 54 had a known human ortolog. The major inflammatory mediators, such as IL-1-6-8, TNFa, NFkB, ETS2, PTGS2, PTX3, CXCL18, KYNU, and CLEC4E were similarly (>2-fold) upregulated in both human and sheep blood.
|
| |
|
| Overall design |
Six healthy human volunteers and six normal sheep blood was incubated with LPS or MPLA for 90 minutes, then the blood was transferred to the Paxgene blood RNA tubes and the gene expression microarrays were scanned with G2556 Microaaray Scanner.
|
| |
|
| Contributor(s) |
Enkhbaatar P, Nelson C, Salsbury J, Carmical JR, Torres KE, Herndon D, Prough D, Luan L, Sherwood ER |
| Citation(s) |
26640957 |
| Submission date |
Aug 31, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Michael Falduto |
| E-mail(s) |
mfalduto@genusbiosystems.com
|
| Phone |
847-291-9602
|
| Organization name |
GenUs BioSystems, Inc.
|
| Street address |
1808 Janke, Unit M
|
| City |
Northbrook |
| State/province |
IL |
| ZIP/Postal code |
60062 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (18)
|
| GSM1865240 |
whole blood, LPS treated, Volunteer 2 |
| GSM1865241 |
whole blood, MPLA treated, Volunteer 2 |
| GSM1865242 |
whole blood, PBS treated, Volunteer 2 |
| GSM1865243 |
whole blood, LPS treated, Volunteer 3 |
| GSM1865244 |
whole blood, MPLA treated, Volunteer 3 |
| GSM1865245 |
whole blood, PBS treated, Volunteer 3 |
| GSM1865246 |
whole blood, LPS treated, Volunteer 4 |
| GSM1865247 |
whole blood, MPLA treated, Volunteer 4 |
| GSM1865248 |
whole blood, PBS treated, Volunteer 4 |
| GSM1865249 |
whole blood, LPS treated, Volunteer 5 |
| GSM1865250 |
whole blood, MPLA treated, Volunteer 5 |
| GSM1865251 |
whole blood, PBS treated, Volunteer 5 |
| GSM1865252 |
whole blood, LPS treated, Volunteer 6 |
| GSM1865253 |
whole blood, MPLA treated, Volunteer 6 |
| GSM1865254 |
whole blood, PBS treated, Volunteer 6 |
|
| This SubSeries is part of SuperSeries: |
| GSE72559 |
Comparison of gene expression by sheep and human blood stimulated with the TLR4 agonists lipopolysaccharide and monophosphoryl lipid A |
|
| Relations |
| BioProject |
PRJNA294326 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72557_RAW.tar |
54.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...