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Series GSE7257 Query DataSets for GSE7257
Status Public on Apr 10, 2007
Title Laser capture-microarray analysis of Lim1 mutant kidney development.
Organism Mus musculus
Experiment type Expression profiling by array
Summary The Lim1 gene has essential functions during several stages of kidney development. In particular, a tissue specific knockout in the early metanephric mesenchyme results in the formation of the earliest nephron precursor, the renal vesicle, but failure of this structure to progress to the next stage, the comma shaped body. To better understand the molecular nature of this developmental arrest we used a laser capture microdissection-microarray strategy to examine the perturbed gene expression pattern of the mutant renal vesicles. Among the genes found differently expressed were Chrdl2, an inhibitor of BMP signaling, the pro-apoptotic factor Bmf, as well as myob5, an atypical myosin which modulates chemokine and transferring signaling, and pdgfr1, which is important in epithelial folding. Of particular interest, the microarray data indicated that the Dkk1 gene, which encodes an inhibitor of Wnt signaling, was downregulated nine fold in mutants. This was confirmed by in situ hybridizations. It is interesting to note that Lim1 and Dkk1 mutant mice have striking similarities in phenotype. These results suggest that the Dkk1 gene might be a key downstream effector of Lim1 function.
Keywords: Lim1 mutant, kidney development, gene expression profile comparison
Overall design We compared gene expression profiles of wild type and mutant Lim1 renal vesicles, which were isolated from E12.5 kidneys by laser capture microdissection. Nine biological independent samples were examined, with four mutant and five wild type. Each sample included approximately 30-50 renal vesicles. Detailed protocols are described at
Contributor(s) Potter S, Hartman HA, Kwan KM, Behringer RR, Patterson LT
Citation(s) 17610272
Submission date Mar 13, 2007
Last update date Feb 11, 2019
Contact name Steven Potter
Organization name Children's hospital Medical Center
Department Developmental Biology
Lab Potter
Street address 3333 Burnet Ave.
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM174757 renal_vesicle_wild_type_1
GSM174758 renal_vesicle_wild_type_2
GSM174759 renal_vesicle_wild_type_3
BioProject PRJNA98013

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7257_RAW.tar 78.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file

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