|
| Status |
Public on Nov 01, 2015 |
| Title |
Kinetic analysis of T cell anergy induction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
T cell receptor(TCR) engagement in the absence of costimulation leads to a state of T cell tolerance known as anergy. Anergy induction requires new protein synthesis since it is inhibited by cycloheximide.
In this experiment, we tried to figure out kinetic properties of the gene expression in anergy induction phase and the subsequent anergy maintenance phase.
|
| |
|
| Overall design |
A.E7 cell, a murine CD4(+) T cell clone, was stimulated with anti-TCR antibody for 0, 2, 4, and 6hrs followed by RNA extraction. For the maintenance phase, A.E7 was stimulated for 16hrs and rested for 5days before RNA extraction.
|
| |
|
| Contributor(s) |
Choi K, Yang J, Schwartz RH |
| Citation(s) |
26507712 |
| Submission date |
Sep 04, 2015 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Kyungho Choi |
| Organization name |
Seoul National University College of Medicine
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Lab of Molecular Immunology
|
| Street address |
103 Daehak-ro, Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
| |
|
| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA294788 |
Less...
More...