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Series GSE7275 Query DataSets for GSE7275
Status Public on Sep 05, 2007
Title Evaluation of murine mast cells derived exosomal RNA versus their parental cells MC/9.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Exosomes are vesicles of endocytic origin released by many types of cells into the extracellular environment. In an attempt to further examine the exosome-mediated cellular communication, we show that exosomes from a mouse mast cell line (MC/9), exosomes from primary bone marrow derived mast cells, and exosomes from a human mast cell line (HMC-1) contain RNA but not DNA.
Microarray assessments of exosome-derived RNA revealed that these vesicles contain mRNA from approximately 1200 genes, many of which are unique and not present in the cytoplasmic RNA pool in the donor cell.
Keywords: Exosomal RNA versus their parental cells, MC/9
 
Overall design Exosomes were prepared from the supernatant of MC/9 cells by differential centrifugations and filtration. RNA was isolated from the exosomes and their parental cells using Trizo. The microarray experiments were performed by SweGene (www.swegene.org/) according to Affymetrix microarray DNA chip analysis. The experiment was performed in quadruple samples. ExoRNA1, ExoRNA2, ExoRNA3, and ExoRNA4 for the exosomes samples and Mast_cells1, Mast_cells2, Mast_cells3, and Mast_cells4 for the MC/9 cells.
 
Contributor(s) Valadi H, Lötvall J
Citation(s) 17486113
Submission date Mar 15, 2007
Last update date Feb 11, 2019
Contact name Hadi Valadi
E-mail(s) hadi.valadi@gu.se
Phone +46(0)704561604
Organization name University of Gothenburg
Department Dept. of Rheumatology and Inflammation Research
Street address Guldhedsgatan 10A
City Gothenburg
ZIP/Postal code 405 30
Country Sweden
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM175530 Exosomal RNA, biological rep1
GSM175531 Exosomal RNA, biological rep2
GSM175532 Exosomal RNA, biological rep3
Relations
BioProject PRJNA97983

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7275_RAW.tar 24.9 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file
Processed data included within Sample table

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