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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 05, 2007 |
| Title |
Evaluation of murine mast cells derived exosomal RNA versus their parental cells MC/9. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Exosomes are vesicles of endocytic origin released by many types of cells into the extracellular environment. In an attempt to further examine the exosome-mediated cellular communication, we show that exosomes from a mouse mast cell line (MC/9), exosomes from primary bone marrow derived mast cells, and exosomes from a human mast cell line (HMC-1) contain RNA but not DNA.
Microarray assessments of exosome-derived RNA revealed that these vesicles contain mRNA from approximately 1200 genes, many of which are unique and not present in the cytoplasmic RNA pool in the donor cell.
Keywords: Exosomal RNA versus their parental cells, MC/9
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| Overall design |
Exosomes were prepared from the supernatant of MC/9 cells by differential centrifugations and filtration. RNA was isolated from the exosomes and their parental cells using Trizo. The microarray experiments were performed by SweGene (www.swegene.org/) according to Affymetrix microarray DNA chip analysis. The experiment was performed in quadruple samples. ExoRNA1, ExoRNA2, ExoRNA3, and ExoRNA4 for the exosomes samples and Mast_cells1, Mast_cells2, Mast_cells3, and Mast_cells4 for the MC/9 cells.
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| Contributor(s) |
Valadi H, Lötvall J |
| Citation(s) |
17486113 |
| Submission date |
Mar 15, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Hadi Valadi |
| E-mail(s) |
hadi.valadi@gu.se
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| Phone |
+46(0)704561604
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| Organization name |
University of Gothenburg
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| Department |
Dept. of Rheumatology and Inflammation Research
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| Street address |
Guldhedsgatan 10A
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| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA97983 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7275_RAW.tar |
24.9 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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